[ccp4bb] Only Five Weeks left to apply for the BNL-Biology and NSLS Course on Synchrotron Data Collection

2008-01-01 Thread Robert Sweet
We will close applications at 8AM EST Monday 4 February 2008 for RapiData 2008, the tenth offering of our popular course: Rapid Data Collection and Structure Solving at the NSLS: A Practical Course in Macromolecular X-Ray Diffraction Measurement The course will be held 6-11 April 200

Re: [ccp4bb] Refmac 5.4.0066 versus Refmac 5.4.0034

2008-01-01 Thread Garib Murshudov
Hi I usually add extra keywords in the "developers option" section of the interface. I hope we will soon change the interface ti suite current and the next version of the program. If unobserved reflections are present in the mtz file with missing reflection flag then for map coefficients

Re: [ccp4bb] Refmac 5.4.0066 versus Refmac 5.4.0034

2008-01-01 Thread Mark A Saper
Garib, Many thanks for pointing out the differences. A couple of more questions: 1. Could you tell me how 5.4.0033 by default handled the free and missing reflections in map calculation. Also, is the ccp4i interface suitable for the latest version of refmac --- I add the extra keywords

Re: [ccp4bb] Plz suggest me

2008-01-01 Thread Gerard DVD Kleywegt
We have crystallized a cyanobacterial phycoerythrin which having two (alpha subunit) in an assymetric unit and held together by only three hydrogen bonds. When we compare the A and B molecule the RMSD is 1.32. because they same sequences then how it is possible to that two molecules are deviated

[ccp4bb] Refmac 5.4.0066 versus Refmac 5.4.0034

2008-01-01 Thread Mark A Saper
Thanks for those pointing out my cut and paste error. Below are the correct numbers. The first term of the formfactor is almost twice as high compared to the other version. I suspect it is because the newer version is using the wavelength in the calculation, and the old version didn't.

Re: [ccp4bb] Plz suggest me

2008-01-01 Thread Kornelius Zeth
could you please be more specific how you refined the structure, what program have you been using and which restraints did you set for the two independent molecules. Best wishes Kornelius On Tue, 1 Jan 2008 11:42:40 + "Md. Imtiyaz Hassan" <[EMAIL PROTECTED]> wrote: > > > Dear freinds

[ccp4bb] Plz suggest me

2008-01-01 Thread Md. Imtiyaz Hassan
Dear freinds We have crystallized a cyanobacterial phycoerythrin which having two (alpha subunit) in an assymetric unit and held together by only three hydrogen bonds. When we compare the A and B molecule the RMSD is 1.32. because they same sequences then how it is possible to that two molec