The review cited below is a great source of ideas to help you get your
ligand in your crystals
Hassell, A et al
Acta Crystallogr D Biol Crystallogr. 2007 Jan;63(Pt 1):72-9.
Crystallization of protein-ligand complexes.
Hopefully it may help you!
Dave
On 11/12/2007, Schubert, Carsten [PRDUS] <[EM
My apologies - previous announcement went out with a wrong subject line.
N
We are pleased to announce the CCP4 workshop titled
"CCP4 school: From data processing to structure refinement and beyond"
which will take place on 23-28 of May 2008 at the Advanced Photon Source
(APS) near Chicago.
T
We are pleased to announce the CCP4 workshop titled
"CCP4 school: From data processing to structure refinement and beyond"
which will take place on 23-28 of May 2008 at the Advanced Photon Source
(APS) near Chicago.
The aim of this inaugural workshop in the USA will be to cover all areas
involv
Hello all,
We are a small equipment company in Northern California that finds itself
with a complete Rigaku crystallography system. We are entertaining
REASONABLE offers for the system (RU-H3R rotating anode generator, R-Axis
IV and R-Axis IV++ detectors, Osmic Mirror Plus/Plus, X-Stream 2000
You could stain your crystals directly with Sybr Gold, which will
fluoresce upon binding nucleic acids, or you can visualize both protein
and RNA on a silver-stained SDS-PAGE gel.
Kevin
Rongjin Guan wrote:
> Dear All
>
> I am trying to co-crystallize a protein and dsRNA complex, and
> looking f
If you have a fluorescence microscope, you could try soaking SYBR-Gold
dye into your crystals.
Kettenberger, H., and Cramer, P. (2006). Fluorescence detection of
nucleic acids and proteins in multi-component crystals. Acta Crystallogr
D Biol Crystallogr 62, 146-150.
Alan
Rongjin Guan wrote:
>
We will shortly be installing a new HP computer for protein crystallography
calculations and e-map fitting.
The operating system is OS X10.5.
Has anyone advice on installing programs O, COOT, CCP4 etc on this system?
James Phillips
Duke University Medical Center
Hi,
we are still in the process to establish a new X-Ray facility and
would like to use small-angle X-ray scattering (SAXS) in house. The
goal is to study protein-protein complexes of ECM proteins.
Can anybody share some experiences on this topic?
thank you in advance
joerg
J
A slight variation on this theme is to use 0.5% toluidine blue (in water) as a
stain. A 2 to 5 minute soak followed by destaining in hot water for a few
minutes is all that is required.
On Tue, 11 Dec 2007 12:14:27 -0600
Paul Paukstelis <[EMAIL PROTECTED]> wrote:
Rongjin,
This
On Tue, 2007-12-11 at 13:00 -0500, Rongjin Guan wrote:
> Dear All
>
> I am trying to co-crystallize a protein and dsRNA complex, and looking
> for methods
> to detect RNA in the crystals. I am thinking about using Mass
> Spectrum, Nanodrop to
> measure OD280/260, etc, but I wonder if ther are som
Dear colleagues,
please find below the programme for the User Group satellite
meeting of the CCP4 Study Weekend which will take place
on Thursday 3rd January 2008.
15:15 Gwyndaf Evans "Status of the MX Beamlines at Diamond"
15:45 Andy Thompson "Progress and first results from MX at Soleil"
16:
Rongjin,
This may not work for you if your RNA is very small, but I have used the
coomassie stain described here:
http://newjournal.kcsnet.or.kr/main/j_search/j_download.htm?code=B021105
to stain dissolved complex crystals run on SDS-PAGE gels. I have found
that it efficiently stains nucleic aci
Dear All
I am trying to co-crystallize a protein and dsRNA complex, and looking for
methods
to detect RNA in the crystals. I am thinking about using Mass Spectrum,
Nanodrop to
measure OD280/260, etc, but I wonder if ther are some more sensitive and
reliable ways.
The crystals are grown in hig
Simon,
solubilize your ligand in DMSO so it is maximally concentrated, 100mM works
fine. Add enough compound to achieve 2-3 fold excess to your protein, mix and
set up. Make sure your final DMSO concentration is ~3%, otherwise chances are
you might harm your protein. If you cannot achieve a hig
Hi all,
I have one ligand which is insoluble in water, and I would like to
co-crystallize it with my protein. Is there any other method except for
dissolving it in DMSO ?
Thanks
Simon
Crystallographic Community:
It is with deep regret that I must report the untimely passing of a valued
member of the crystallographic community, Professor John J. Stezowski. John
was a Professor of Chemistry at the University of Nebraska from 1991 up until
the time of his passing. John began his
You could, for example, try and increase the precipitant concentration
after the crystals have grown. This might stabilise them even at room
temperature and may also improve their quality.
If you cover your trays with tape, you can easily exchange the reservoir
solution with a syringe without
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