Hey, Engin,
I saw this instrument at the Protein Society meeting this summer. Looks
impressive. Be aware that even though the volume will be significantly
smaller, the concentration needed to get a measurable amount of heat will
probably be higher (the Microcal rep I talked to confirmed this t
Dear Engin,
We have just purchased a new iTC200 but are still waiting
for it to arrive. I can update you when we've done some
experiments in the new year to compare with the old
Microcal.
As you say we were attracted due to the reported low
sample useage. You will have to realise though tha
Fellow crystallographers and biochemists,
This will be off topic. Does anyone have any experience with the new
ITC Microcal is selling (itc200)? They claim that itc200 requires one
seventh the sample volumes. We're especially interested in an
equipment that would require less sample withou
Hi,
Why is it that you are so reluctant to identify this compound
as Ser-Gly? Its fit to the density is great.
To wax historical, we saw unexpected density in the active site
of "apo" Thermolysin. It appeared to be Val-Ala, but with refinement
it developed into Val-Lys. It happens that
We are offering RapiData 2008, the tenth offering of our popular course:
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The course will be held 6-11 April 2008. Students could be at any level
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Phoebe,
In addition to all other comments I like to add that have had several
cases when crystals would not take/like any cryo agent at all. What I
did is added 1% glycerol in the crystallization solution. That did not
change crystallizability of any of the proteins I worked with, but as a
result
Joyce,
In the US, we have often purchased HA compounds from Strem (http://
www.strem.com). They make a lot of different organometallic
compounds as well.
Michael
R. Michael Garavito, Ph.D.
Professor of Biochemistry & Molecula
Many thanks to all who replied. The answers were remarkably varied -
see below.
My own two bits worth - vitrification of mother liquor doesn't always
lead to a nice, low-mosaicity, ice-free crystal freeze in our hands,
although we're not willing to sacrifice a statistically significant
number
Been Googling a bit, Methyl mercuric acetate is sold by Chemos in Germany,
perhaps they have the others too.
http://www.chemos-group.com/
Flip
From: CCP4 bulletin board [mailto:[EMAIL PROTECTED] On Behalf Of Joyce,
Gordon M.(NIH/NIAID) [F]
Sent: Thursday, December 06, 2007 4:32 PM
To: CCP4
I am trying to locate a source for the following heavy atom compounds.
Trimethyl lead acetate
Triethyl lead acetate
Methylmercury acetate
Any suggestions would be greatly appreciated. I have checked the usual
suspects but haven't found anything as yet.
Yours,
M. Gordon Joyce,
Vi
A permanent protein crystallisation position at AstraZeneca in Boston, USA,
advertised here earlier, is still vacant. The position is within Infection
research and would involve crystallisation of antibacterial target proteins as
part of an increased strategic interest in antibiotics. The positi
This result means that you need to use TLS refinement and it may
improve your statistics considerable.
Garib
On 6 Dec 2007, at 11:27, Ronan Keegan wrote:
From: Rafael Couñago <[EMAIL PROTECTED]>
Date: 6 December 2007 04:58:51 GMT
To: <[EMAIL PROTECTED]>
Subject: [ccp4]: iso - anisotropic
--- Begin Message ---
Hi,
I had a very curious result when doing a round of isotropic B factor
refinement on Refmac5.
After iso B my R is 19.2 and Rf 24.3. My resolution is 1.95, what does
not justify anisotropic b-factor refinement. However I did it by mistake
and it dropped my R to 17.8
Posted on behalf of Rik Wierenga:
*'
Applications are now welcome for the next spring BIOXHIT/BCO protein
crystallography course in Oulu, Finland, entitled "The CCP4 structure
determination pipeline", April 21-25, 2008.
Briefly, it i
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