The main reason was related to absorption. If you didn't completely bathe
the crystal in the xray beam, then the diffracting volume of the crystal
would be different during the data collection, and thus, scaling would be
inaccurate, especially when there was radiation damage. This was
especially tr
It probably goes back to the days of using a single-counter diffractometer
where one didn't have multiple Bragg reflections on an image or film pack.
That is, each reflection was collected by itself. Even in a small molecule
crystal data collection nowadays, it would not hurt to have the crystal
c
Jorge,
You said,
I remember one former good (small molecule ?) crystallography book
with words a kind of this "the crystals should be completely bathed by
the x-ray beam during the whole data collection" and also some other
concerns about beam homogeneity in its cross section. How serious
Hi Deliang,
My own experience with two membrane proteins suggests that His-tag
issues pertaining to soluble proteins also seem to apply to membrane
proteins.
In one case, for example, cleaving the N-terminal His-tag improved the
solubility of the protein dramatically. Interestingly, expre
Concerning what Pierre asked, one point to remember at structure
refinement is to test and probably use TLS tensors. I had one case with
serious anisotropy in the data due a libration axis parallel to a
crystallographic axis. TLS lowered dramatically the R's and maps were neatly
better.
Hi Deliang,
We have crystallized the complex cytochrome b6f with an extension of
His6 (no linker) at the c-terminal of cytochrome f (Stroebel, 2003). The
His extension takes part to the crystal contacts. The electron density
is visible but not very well defined. We have not tried to remove the
Hi there,
I purified a membrane protein with traditional His-tag on the C terminal.
Before crystallization, I wonder how this tag may affect the result. Does
anyone have experience that the removel of this tag may improve the result or
not? or can provide some references which may give some st
Doesn't POLARRFN have this option too (i.e. to print all symmetry related
peaks) ? Maybe not through the gui but most likely in command line mode.
Boaz
- Original Message -
From: Eleanor Dodson <[EMAIL PROTECTED]>
Date: Friday, November 23, 2007 12:37
Subject: Re: [ccp4bb] Summary:
Dear Lucas and All
the jiscmail team are having problems with mail sent to yahoo
accounts. I received this from the jiscmail helpline on 2 Nov.
"We are having issues delivering to Yahoo addresses and are actively
trying to
resolve this as quickly as possible.
On our tests, the JISCmail e
A bit late but I didnt answer in time.
This is one of the cases where I still wheel out ALMN to do the self
rotation. It generates ALL symmetry peaks and allows you to select which
axis you want to take as the polar axis. (ncode = 1 c*, ncode = 2 a*,
ncode = 3 b* and so on)
And the program out
Dear CCP4ers,
I've asked you about symmetry in stereographic projections of self-
rotation functions, because I have in a monoclinic space group with
beta=97 a peak for a NCS 7-fold axis at Phi=83, Psi=90, Kappa=51.4.
In this self-rotation function, calculated with GLRF, the monoclinic
b-a
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