[ccp4bb] PhD studentship in structural biology

2007-11-22 Thread Andrew Sutherland-Smith
Dear CCP4 list, We have a PhD studentship funded by a New Zealand Royal Society Marsden Grant for three years to investigate the structure, functional properties and regulation of an enzyme hypothesised to be important for cellular responses to hypoxia and/or low glucose concentrations,

Re: [ccp4bb] Symmetry of crystallographic stereographic projections?

2007-11-22 Thread Dirk Kostrewa
Hi Ian, many thanks! Apparently, I have to discard now my favorite hypothesis about the position of the 7-fold ... Still, if you know about a good reference, I would really like to read it. Best regards, Dirk. Am 22.11.2007 um 15:28 schrieb Ian Tickle: Hi Dirk Sorry yes you're right

Re: [ccp4bb] Symmetry of crystallographic stereographic projections?

2007-11-22 Thread Ian Tickle
Hi Dirk Sorry yes you're right I'm talking nonsense, c* will be at phi=90; that's what comes of not making a diagram! As far as the description of dyads goes the POLARRFN description is complete. It's only incomplete for higher order axes, so I think the symmetry you hope to find doesn't exist.

Re: [ccp4bb] Anisotropic data - How to deal with ?

2007-11-22 Thread Anastassis Perrakis
On Nov 22, 2007, at 14:43, Kay Diederichs wrote: Pierre Barraud schrieb: Dear all, I am working on a data set which is severely anisotropic, with diffraction limits of 2.6 A along the a* and b* direction but only 3.3 A along the c* direction. I attached a screen shot of the Anisotropic a

Re: [ccp4bb] Symmetry of crystallographic stereographic projections?

2007-11-22 Thread Dirk Kostrewa
Hi Ian, thanks for the POLARRFN pointer - I've read this before, but I didn't find it complete enough ... Shouldn't the c*-axis have Phi=90, since it is c*=axb? I don't see a peak at 180-Phi, but I thought that there might be other symmetry- equivalent peaks that have some rule for Phi, Psi,

Re: [ccp4bb] Anisotropic data - How to deal with ?

2007-11-22 Thread Harry Powell
Hi If you want to integrate anisotropically, Mosflm has allowed it since late in the last Century. However, the last time I mentioned it to Garib he said that he'd prefer to have the data integrated isotropically (even the data that "aren't there becasue they're too weak"). On 22 Nov 2007

Re: [ccp4bb] Anisotropic data - How to deal with ?

2007-11-22 Thread Dirk Kostrewa
Dear Pierre, there is also a "Diffraction Anisotropy Server" at UCLA that does some scaling and truncating of anisotropic data, possibly making your crystallographic life a bit easier ... but I don't have personal experiences with this approach. Have a look at http://www.doe-mbi.ucla.edu/~

Re: [ccp4bb] Symmetry of crystallographic stereographic projections?

2007-11-22 Thread Ian Tickle
Hi Dirk There's a useable (though slightly incomplete) description of the symmetry of SRF's in the POLARRFN doc, though this only talks about the symmetry of the stereographic projection itself and then only about the effect of crystallographic dyads. Higher order rotations about z will obviously

Re: [ccp4bb] Anisotropic data - How to deal with ?

2007-11-22 Thread Kay Diederichs
Pierre Barraud schrieb: Dear all, I am working on a data set which is severely anisotropic, with diffraction limits of 2.6 A along the a* and b* direction but only 3.3 A along the c* direction. I attached a screen shot of the Anisotropic analysis (FALLOFF) output graph from SCALA. My questio

[ccp4bb] Symmetry of crystallographic stereographic projections?

2007-11-22 Thread Dirk Kostrewa
Dear CCP4ers, does any of you have a good reference describing the symmetry of crystallographic stereographic projections? There is a lot of literature describing rotational symmetry in Eulerian angular space, but I'm not aware of any for polar angles. In particular, I've calculated a sel

[ccp4bb] Anisotropic data - How to deal with ?

2007-11-22 Thread Pierre Barraud
Dear all, I am working on a data set which is severely anisotropic, with diffraction limits of 2.6 A along the a* and b* direction but only 3.3 A along the c* direction. I attached a screen shot of the Anisotropic analysis (FALLOFF) output graph from SCALA. My question is : what is the best s

Re: [ccp4bb] Installation problem on Fedora 8

2007-11-22 Thread Anastassis Perrakis
If I may make a general note: I would suggest to everybody not to install anything as the 'root' user, unless these are system utilities. Most if not all packages can be installed under a user account, or under another dedicated account (eg Software) and be made available to others. This ha