[ccp4bb] Apple X11 10.5

2007-10-27 Thread William Scott
Several people have emailed me about X11 in OS X 10.5. Because I pre- ordered it, I haven't received it yet, so I have only gotten to enjoy the migraines vicariously. Hence, i can't really help. Meanwhile, this looks informative: http://lists.apple.com/archives/X11-users/2007/Oct/msg00065.h

Re: [ccp4bb] X-tallography on OS X aka 10.5 Leopard

2007-10-27 Thread William Scott
Juergen Bosch wrote: > @Bill, > > how about your experience with 10.5 ? Dear Apple Customer, Apple is pleased to report that a shipment for the following order is on its way to you. The following products shipped on 10/26/2007. Product # Product Description QtyE

[ccp4bb] Is phophorylation possible in E. coli expression system?

2007-10-27 Thread Mark A Saper
Tyrosine phosphorylation has been known in many bacteria since the late 90s. See for example, Ilan, O., Bloch, Y., Frankel, G., Ullrich, H., Geider, K. & Rosenshine, I. (1999). Protein tyrosine kinases in bacterial pathogens are associated with virulence and production of exopolysacchari

Re: [ccp4bb] Is phophorylation possible in E. coli expression system?

2007-10-27 Thread Dima Klenchin
However, if your protein is not a protein tyrosine kinase, you may check your western condition. Yes, this is an essential control. Use lots of Lambda protein phosphatase to "dephosporylate" your protein and us the resulting material as a negative control in Western. My experience is that pho

[ccp4bb] Summary: Is phophorylation possible in E. coli expression system?

2007-10-27 Thread Narayanan Ramasubbu
Dear All: Thanks for all your replies to this. Phosphorylation is possible in E. coli. One article of particular interest is Mol Cell Proteomics 2007 Eprint by Macek B, Gnad, F, Soufu B, Kumar C, Olsen JV, Mijakovic, I and Mann M. Many others have pointed out that it is possible and many likely

[ccp4bb] X-tallography on OS X aka 10.5 Leopard

2007-10-27 Thread Juergen Bosch
Hi all, just a quick draft report on 10.5 and our beloved programs. Upgraded an existing 10.4.10 ( by the way you will need ~5 GB to upgrade your existing system), no clean install. Coot 0.31 does not work (need to upgrade anyway, more sometime this weekend, seems to have troubles with libti