Dear Colleagues,
We would like extend an invitation to you for the 3rd annual Ohio Valley
Crystallography and Biophysics Symposium (now with more biophysics!), to be
held this year at the University of Toledo, Ohio on Friday, November 9th. In
addition to talks on macromolecular structure determ
Dear Colleagues,
It is with sorrow I report the passing of Gerard (Gerry) Bunick on September
19, 2007 in Oak Ridge, Tennessee after an 11 year struggle with cancer. In
addition to other significant research, Gerry was one of the architects of
the resurgence in neutron protein crystallography.
On Fri, 19 Oct 2007, Kay Diederichs wrote:
Acta Cryst. (2004). D60, 2196-2201
Direct incorporation of experimental phase information in model refinement
P. Skub?k, G. N. Murshudov and N. S. Pannu
the refmac documentation(s) mention Rice, MLHL, etc., but i didn't see
explicit mention of SAD - i
I'd try to
1) make sure your data reduction is optimal - try at least one other program,
and not only your favourite one!
2) as this is SAD: use SHARP to get the best experimental phases, plus density
modification for a map without model bias
3) incorporate the experimental phase information int
Mark,
The bulk solvent model in CNS 1.2 is now perfectly stable at low
resolution. No need
to fix the solvent parameters anymore.
Axel
Mark A. White wrote:
Joe,
1) The bulk solvent models in CNS and REFMAC are not stable at low
resolution. Fixing the bulk solvent B-factor can improve th
Merge in the lower symmetry space group you mentioned and refine with a
twinning operator.
xtriage can tell you what the twinning operator is and phenix.refine can
do the twinned refinement.
In my experience, these kinds of stats almost always indicate an
incorrect symmetry choice somewhere
Joe,
1) The bulk solvent models in CNS and REFMAC are not stable at low
resolution. Fixing the bulk solvent B-factor can improve this behavior.
2) With low resolution data model-bias becomes a serious issue when
working with a MR solution. I have found that DM (with or withou NCS)
can improve l
These problems are horrible!
1) I usually try to run refinement using the exptl phases as restraints.
It is worth getting the best possible phases before you start - DM with
66% solvent should make a great improvement..
Then these cam be used as restraints for the refinement, and your FWT
m
Hi Joe,
have you tried to process the data in different programs ? This can
sometimes make a dramatic difference, being completely unbiased I would
recommend XDS over Mosflm or HKL2000. Was your data carefully collected
? How many rejections, overlaps do you have ?
Juergen
--
Jürgen Bosch
U
Hi all,
We have data sets for a protein-RNA complex at 3.2 A resolution. . The
data belong to the space group I4122 and contains one molecule of
Protein-RNA complex (300AA and 16nt; 66% solvent content) in the asym
unit. We have managed to get phases using Se-SAD and the present model
contains 60%
Dear all,
I'm looking for a pdf of the paper
Atomic structure and specificity of bacterial periplasmic receptors
for active transport and chemotaxis: variation of common themes
Florante Quiocho, P Ledvina
Mol Microbiol. 1996 Apr: 20(1)17-25
Our library only has electronic access from 1997 an
> Dear SRS Users,
>
> Applications are now invited for beam-time in allocation period 50 (April -
> December 2008) on PX station 10.1 at the SRS. The deadline for submission of
> proposals is Thursday 1st November.
>
> Further details on how to apply for beamtime can be found here :-
> http:/
User error I'm afraid.
You are using the 'add domain' button in the GUI, you should be using
the 'add operator' button instead.
Kevin
Bernhard Rupp wrote:
Thanks to Pete and Juergen - seems that the
GUI sticks the offending
domain 1 -
domain 2 -
line in the script. Both the linux and
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