To add to this list you might also try the Pharmacia resin instead of
Qiagen's (or the other way round, depending on what you are using now).
Qiagen use Ni-NTA while Pharmacia's resin is IDA which chelates Ni in a
different way. During my PhD this made an all-or-nothing difference with a
protein t
The presence of a His-tag in the sequence does not automatically mean that
the protein will bind to IMAC resin. Since your protein is so huge and since
the His-MBP-fusion does not work any better than the His-fusion I would
hazard that your protein is highly aggregated in solution, to the extent
th
Hi,
This has been discussed often. You can look back through old posts or also
look at troubleshooting pages in purification manuals that come with the some
of the products like Qiagen Ni-NTA beads, etc.
Briefly, you can try the following:
a) Play around with loading rate, i.e., go down to m
Dear all,
I am trying to express a 150 kd protein in E coli. I have it in two
constructs, one with pmal-his and other with only his tag at N terminus. The
full length protein can be detected both by sds and western using anti-his
(190kd and 150kd respectively) but strangely neither binds to his-co
Dear All,
I read in SFALL docu that a "2- or 5-Gaussian"
approximation for SFs is used - I quote from SFALL docu:
begin
a1*exp(-b1*s*s) + a2*exp(-b2*s*s) - 2 Gaussian approximation
Hi,
I am looking to take a pdb and make several altertnate conformers of the whole
protein molecule. Is there an easy way to do this?
Thanks,
John Bruning
On Wed, October 10, 2007 11:50 am, Bryan W. Lepore wrote:
> On Wed, 10 Oct 2007, Jim Pflugrath wrote:
>> It has come to my attention that the wavelength of a Copper Kalpha may
>> have changed over the years. At least this appears to be true if you
>> look at the International Tables.
>
> the 'natu
On Wed, 10 Oct 2007, Jim Pflugrath wrote:
It has come to my attention that the wavelength of a Copper Kalpha may
have changed over the years. At least this appears to be true if you
look at the International Tables.
the 'natural' isotopic distribution must have changed, as it has with the
bi
It has come to my attention that the wavelength of a Copper Kalpha may
have changed over the years. At least this appears to be true if you look
at the International Tables.
What is the currently approved wavelength in Angstrom of a Copper Kalpha
X-ray produced by a X-ray generator with a cop
