Hi
Bill Scott used a RNA fragment based MR approach in his paper on Ribozyme
structure.
The Structural Basis of Ribozyme-Catalyzed RNA Assembly
Michael P. Robertson and William G. Scott
Science 16 March 2007:
Vol. 315. no. 5818, pp. 1549 - 1553
In our lab we had an interesting time with the tw
I would use a very general definition for "solvent",
including disordered detergent and lipids.
As you know in many cases ordered detergents and lipids
have been modeled in the coordinates, so they are part of
the model not the solvent. In some cases I think waters
should be included in the model
Dear colleagues,
in estimating the solvent content of membrane protein crystals it
would only seem reasonable that micelle size should also be taken into
account. Depending on the aggregation number and MW of a given
detergent, the concentation of detergent used, and the buffer
conditions
The paper below can put things in perspective a little bit.
Bernstein and Hol (1997) Acta Crystallogr D Biol Crystallogr. 53(Pt
6):756-64.
Probing the limits of the molecular replacement method: the case of
Trypanosoma brucei phosphoglycerate kinase.
best wishes
Savvas
I am pretty sure the work you are referring to is R. B. G. Ravelli et.
al. JSR 2007
http://dx.doi.org/10.1107/S0909049506043111
the article is open access, so anyone anywhere can click on the above
link and read it.
Basically, they used EM specimen preparation methodologies to embed a
lysozym
a quick summary on 'the worst MR probes'
Pierre Rizkallah:
X-ray Structure Solution of Amaryllis Lectin by Molecular Replacement
with Only 4% of the Total Diffracting Matter.
L. Chantalat, S.D. Wood, P.J. Rizkallah, C.D. Reynolds (1996).
Acta Crystallographica, D52, pp. 1146-1152
Poul Nissen:
Ni
Hi all,
along the lines of a recent discussion:
At the final stages of the refinement of a structure which a whole bunch of
ncs (2x4chains) at 2.2A resolution in P1, I run into the following very
annoying and persistent problem. The Ramachandran plot shows a whole bunch
of ugly outliers. While some
Sorry - didn't see this - time spent on MR solution of the
Phe-tRNA:EF-Tu:GDPNP structure was about three months (but this was 1994
with slow computers, two trips per year to the synchrotron etc.)
Poul
> To avoid excessive excitement potentially caused by such a list, people
> should also indica
An old example:
The Phe-tRNA:EF-Tu:GDPNP ternary complex - three complexes per asymmetric
unit, C2 space group, degenerate three-fold NCS (three different tRNA
conformations in the trimer). 40 - 2.8 Å resolution native data (about 78%
complete...) used for MR. Straight-forward placement of three EF
