Re: ccp4bb on new site

Unfortunately, It appears that JISCMAIL is using the outdated LISTSERV software to run it's mailing lists, so there is not much hope of getting such things as the [ccp4bb] subject tag and "reply to sender" features back :-( Morten -- Morten Kjeldgaard, asc. professor, MSc, PhD Department

Re: ccp4bb on new site

Hi all, just realised that in the new ccp4bb setup (using various mail programs such as thunderbird, mail, webpine), the actual sender does not appear in the list of addressees, not even using "reply to all". I personally sometimes find it more appropriate to respond only to the person asking

Re: ccp4bb on new site

Douglas L. Theobald wrote: On Jan 19, 2007, at 4:20 PM, Steve Lane wrote: Agreed. If at all possible, please resume use of the [ccp4bb] Subject: tag. I'll add another vote for the [ccp4bb] tag. -- Steve Lane System, Network and Security Administrator Doudna Lab Biomolecular Structure a

Re: ATP analogues for co-crystallization

for ground state ATP analogues use AMP-PNP (or AMP-PCP) or ADP in complex with beryllium fluoride. In some circumstances ATP-gamma-S will work too. It's less resistant to hydrolysis, so it's a bad idea for many enzymes, but the resulting active site geometry can be different depending on whic

Re: How to find a third or maybe fourth molecule in MR?

Hi Jenny, As you have a trigonal/hexagonal spacegroup, have you eliminated the possibility of twinning? This can create ghosts in the density... Cheers, James

How to find a third or maybe fourth molecule in MR?

Hi,Dear all Sorry to bother you all, but I'm kinda of stuck here now and I really need some advices. Briefly overview,my mutation protein is 10kd,with cell size 137.200 137.20086.68290.00090.000 120.000.We are able to find two molecules now and when we look at the map,these two m

Re: ATP analogues for co-crystallization

Hi Zhiyi, for ground state ATP analogues use AMP-PNP (or AMP-PCP) or ADP in complex with beryllium fluoride. transition state like analogues can be formed with ADP in complex with aluminium fluoride or magnesium fluoride. ADP - nitrate has also been used as a transition state analogue, hope this

Re: ATP analogues for co-crystallization

Dear Zhiyi, Also, you can have look in the literature to ADP-AlF4 and ADP-BeF3 complexes. Bests of luck! Ruben 2007/1/20, [EMAIL PROTECTED] <[EMAIL PROTECTED]>: Hi, Zhiyi, Try AMP-PNP, Sigma catalog A2647. Good luck, Hidong *ZhiYi Wei <[EMAIL PROTECTED]>* Sent by: CCP4 bulleti