Re: [Bioc-devel] Does it matter whether I pool technical replicates for edgeR

2012-12-06 Thread Kasper Daniel Hansen
On Thu, Dec 6, 2012 at 2:29 PM, Ryan C. Thompson wrote: > Hi all, > > I'm working with an RNA-seq dataset where every biological sample has two > technical replicates. Is important for me to merge the technical replicates > so that my count matrix has exactly one column per biological sample, or i

[Bioc-devel] Does it matter whether I pool technical replicates for edgeR

2012-12-06 Thread Ryan C. Thompson
Hi all, I'm working with an RNA-seq dataset where every biological sample has two technical replicates. Is important for me to merge the technical replicates so that my count matrix has exactly one column per biological sample, or is it ok to leave them separate? My worry would be that leavin