Re: [Bioc-devel] From Biostring matching to short read mapping

2019-11-09 Thread Wei Shi
computational cost consideration. Thanks for considering Subread and good luck for your project. Wei From: Bhagwat, Aditya Sent: Saturday, November 9, 2019 8:06 pm To: Pages, Herve; bioc-devel@r-project.org Cc: Wei Shi; Michael Stadler (michael.stad...@fmi.ch

Re: [Bioc-devel] Question about external algorithms to Bioconductor package

2017-11-26 Thread Wei Shi
you can provide your command and screen output. But there are certainly other Bioconductor packages that can do proper alignment for you. Wei Wei Shi, PhD Laboratory Head The Walter and Eliza Hall Institute of Medical Research Melbourne, Australia From: Ioannis Vardaxis

Re: [Bioc-devel] Question about external algorithms to Bioconductor package

2017-11-26 Thread Wei Shi
.; Ryan Thompson Cc: bioc-devel@r-project.org; Wei Shi Subject: Re: [Bioc-devel] Question about external algorithms to Bioconductor package I think that generally Rsubread is 'fast' so you might make sure that there are not obvious problems, e.g., aligning reads to the wrong reference;

[Bioc-devel] Experimental data package 'seqc'

2014-11-18 Thread Wei Shi
We have created a new experimental data package called 'seqc'. It includes gene-level read count data generated by the SEQC (SEquencing Quality Control) project, which is the third stage of the well-known MAQC project (a US FDA initiative). The SEQC/MAQC-III Consortium produced benchmark RNA-seq

Re: [Bioc-devel] subread-buildindex fails on genome with many scaffolds?

2013-11-26 Thread Wei Shi
Hi Ryan, It seems you are using a quite old version of Subread. The latest version of SourceForge Subread is 1.4.2 and the latest version of bioc Rsubread is 1.12.3. These indexing building issues had already been fixed. Cheers, Wei On Nov 27, 2013, at 12:12 PM, Ryan C. Thompson wrote: > Actu

Re: [Bioc-devel] Problem in asBam from Rsamtools

2013-06-04 Thread Wei Shi
that this created some problem to the I/O > also because during the run of Rsubread there were many other I/O processes > running. > I run again Rsubread and I did not got any error > Thanks for the help > Raf > > On 6/2/13 1:22 AM, Wei Shi wrote: >> Dear Raf, >> >&

Re: [Bioc-devel] Problem in asBam from Rsamtools

2013-06-01 Thread Wei Shi
Dear Raf, As Martin pointed, that line seems to be the concatenation of two records. But the second record is incomplete (it doesn't have the read identifier). It seems more likely to be a file system problem rather than Rsubread problem. Could you please also provide the line before the proble

Re: [Bioc-devel] Rsubread build error

2013-03-29 Thread Wei Shi
and the latest Rsubread version is 1.9.7. Hope it now works on george2. The version of R I'm using is R3.0.0rc r62427. Best regards, Wei On Mar 29, 2013, at 3:08 PM, Martin Morgan wrote: > On 03/28/2013 08:36 PM, Wei Shi wrote: >> Dear Bioc core team, >> >> Our R

[Bioc-devel] Rsubread build error

2013-03-28 Thread Wei Shi
Dear Bioc core team, Our Rsubead package got a build error on george2, but we could not reproduce the error on our local linux machine. It is successfully built and installed on our linux computer. Is it possible that you can give us more information about what might cause this error? Many tha