Re: [Bioc-devel] lazyData (Kasper Daniel Hansen)

2016-07-30 Thread Kasper Daniel Hansen
Fair enough; I'm aware of this. But loading the annotation package into the environment of a function means reloading it every time I need it, which is pretty often. LazyData is just so much nicer. Best, Kasper On Sat, Jul 30, 2016 at 12:44 PM, Alex Pickering wrote: > I was experiencing simil

Re: [Bioc-devel] lazyData (Kasper Daniel Hansen)

2016-07-30 Thread Alex Pickering
I was experiencing similar issues of long build times with LazyData TRUE so I made it FALSE. You can specify the environment in which data is loaded (it doesn't have to be the global environment). For example, to load the cmap_es data from the ccdata package call this from within a function: util

Re: [Bioc-devel] No Significant hits!

2016-07-30 Thread Sean Davis
Hi, Pabita. This question is better asked on the bioconductor support site ( HTTPS://support.bioconductor.org). However, finding no evidence for differential expression is not uncommon. Whether it is expected or not is hard to comment on without data. Sean On Jul 30, 2016 10:43, "Pabita Basnet"

[Bioc-devel] No Significant hits!

2016-07-30 Thread Pabita Basnet
Hi all I am using DEXSeq to analyse genome wide exon usage in RNA-Seq sample from maize. I have two factors data. One is condition (weedy/weed-free) and thiamethoxam treatment (treated/untreated). Each has 3 replicates. I wanted to see the differential exon usage for weedy versus weed-free, treated