Re: [Freesurfer] Incomplete and poorly formed tracts

2013-03-26 Thread Susan Kuo
Hi Anastasia,
  I did as you recommended and checked the diffusion-to-anatomical
registration, and the overlay of aparc+aseg_mask on FA, and these views
seem to be good. Upon closer inspection, what I find is that there are
incipient 'bits' of all the tracts, but they seem to not have 'grown',
though they are in the proper space (comparing them to good brains that
yielded the full complement of tracts). Is there a configuration in your
TRACULA that controls the growing of the tracts specifically? Perhaps I
should look into that.

  Thank you, btw, for your very prompt reply yesterday- it was much
appreciated!


Sincerely,
Susie Kuo
NIH

On Mon, Mar 25, 2013 at 4:58 PM, Anastasia Yendiki <
ayend...@nmr.mgh.harvard.edu> wrote:

>
> Hi Susan - Good to hear that you get good results for most of your
> subjects. Have you checked the aparc+aseg and the diffusion-to-anatomical
> registration for the subjects that are failing? I'd check the
> aparc+aseg_mask (in the dlabel/diff directory) over the FA map to see if
> there are any holes or misregistration.
>
> a.y
>
>
> On Mon, 25 Mar 2013, Susan Kuo wrote:
>
>  Hi FreeSurfers and Anastasia,   TRACULA is working great for me,
>> generating tracts for a sample of 20 subject
>> brains I'm working with. However, for 3 of the brains, I'm receiving
>> incomplete and poorly formed tracts.
>> I've re-run trac-all at least 2x on each subject in case there was a
>> mistake in my original configuration.
>> However, I am reproducing the same results. Does anybody have an idea why
>> I would see these "spotty" tracts?
>>
>>
>> Thank you for all your help!
>>
>> --
>> Susie Kuo
>> NIH
>>
>>
>>
>
> The information in this e-mail is intended only for the person to whom it
> is
> addressed. If you believe this e-mail was sent to you in error and the
> e-mail
> contains patient information, please contact the Partners Compliance
> HelpLine at
> http://www.partners.org/**complianceline<http://www.partners.org/complianceline>.
>  If the e-mail was sent to you in error
> but does not contain patient information, please contact the sender and
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> dispose of the e-mail.
>



-- 
Susie Kuo

Mediocrity knows nothing higher than itself, but talent instantly
recognizes genius. - Sir Arthur Conan Doyle,* Sherlock Holmes- Valley of
Fear*
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Re: [Freesurfer] Incomplete and poorly formed tracts

2013-03-26 Thread Susan Kuo
Hi Anastasia,

  I see what you mean. I had previously used the dtifit_FA.nii.gz image and
overlaid that with the aparc+aseg+2mm.nii.gz image in /dlabel/diff, using
freeview. I didn't end up with the same images as you, however. Can you
tell me which images you used to obtain this overlay?

  I am going to run bbregister tonight, and I'll let you know how it goes
-- I'm hopeful!

Thank you again!

Susie Kuo
NIH

On Tue, Mar 26, 2013 at 5:35 PM, Anastasia Yendiki <
ayend...@nmr.mgh.harvard.edu> wrote:

>
> Hi Susie - I'm attaching a snapshot from your subject, showing the
> aparc+aseg overlaid on the FA map. The registration has failed. The frontal
> lobe has spilled out of the brain, the white matter has spilled into the
> ventricles.
>
> I strongly recommend using bbregister for the intra-subject registration,
> which is the default in the latest version of trac-all.
>
> Hope this helps,
> a.y
>
>
> On Tue, 26 Mar 2013, Susan Kuo wrote:
>
>  Hi Anastasia,   I did as you recommended and checked the
>> diffusion-to-anatomical
>> registration, and the overlay of aparc+aseg_mask on FA, and these views
>> seem to be
>> good. Upon closer inspection, what I find is that there are incipient
>> 'bits' of all
>> the tracts, but they seem to not have 'grown', though they are in the
>> proper space
>> (comparing them to good brains that yielded the full complement of
>> tracts). Is there a
>> configuration in your TRACULA that controls the growing of the tracts
>> specifically?
>> Perhaps I should look into that.
>>
>>   Thank you, btw, for your very prompt reply yesterday- it was much
>> appreciated!
>>
>>
>> Sincerely,
>> Susie Kuo
>> NIH
>>
>> On Mon, Mar 25, 2013 at 4:58 PM, Anastasia Yendiki <
>> ayend...@nmr.mgh.harvard.edu>
>> wrote:
>>
>>   Hi Susan - Good to hear that you get good results for most of your
>>   subjects. Have you checked the aparc+aseg and the
>> diffusion-to-anatomical
>>   registration for the subjects that are failing? I'd check the
>>   aparc+aseg_mask (in the dlabel/diff directory) over the FA map to
>> see if
>>   there are any holes or misregistration.
>>
>>   a.y
>>
>>   On Mon, 25 Mar 2013, Susan Kuo wrote:
>>
>> Hi FreeSurfers and Anastasia,   TRACULA is working great for
>> me, generating tracts for a sample of 20 subject
>> brains I'm working with. However, for 3 of the brains, I'm
>> receiving incomplete and poorly formed tracts.
>> I've re-run trac-all at least 2x on each subject in case there
>> was a mistake in my original configuration.
>> However, I am reproducing the same results. Does anybody have
>> an idea why I would see these "spotty" tracts?
>>
>>
>> Thank you for all your help!
>>
>> --
>> Susie Kuo
>> NIH
>>
>>
>>
>>
>> The information in this e-mail is intended only for the person to whom it
>> is
>> addressed. If you believe this e-mail was sent to you in error and the
>> e-mail
>> contains patient information, please contact the Partners Compliance
>> HelpLine at
>> http://www.partners.org/**complianceline<http://www.partners.org/complianceline>.
>>  If the e-mail was sent to you in error
>> but does not contain patient information, please contact the sender and
>> properly
>> dispose of the e-mail.
>>
>>
>>
>>
>> --
>> Susie Kuo
>>
>> Mediocrity knows nothing higher than itself, but talent instantly
>> recognizes genius. -
>> Sir Arthur Conan Doyle, Sherlock Holmes- Valley of Fear
>>
>>


-- 
Susie Kuo

Mediocrity knows nothing higher than itself, but talent instantly
recognizes genius. - Sir Arthur Conan Doyle,* Sherlock Holmes- Valley of
Fear*
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contains patient information, please contact the Partners Compliance HelpLine at
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Re: [Freesurfer] Incomplete and poorly formed tracts

2013-03-27 Thread Susan Kuo
Hi Anastasia,
  I'm attaching the graphic I arrived at with the command:

> freeview -tv /dlabel/diff/aparc+aseg.flt.nii.gz -v
/dmri/dtifit_FA.nii.gz

Here, I used the subject I send you (Sa69845.5) for the subjectID. I could
not detect spill over in these images, mostly because the frontal lobe is a
little cut off. Did you perhaps use a different viewer? I'm hoping to be
able to detect these mis-registrations better for myself in the future.
Thanks for all your help!

Sincerely,
Susie Kuo
NIH

On Wed, Mar 27, 2013 at 1:32 AM, Anastasia Yendiki <
ayend...@nmr.mgh.harvard.edu> wrote:

>
> Hi Susie - Everything under dlabel/diff is mapped to DWI space (either
> with flirt or with bbregister, whichever you use). The one in my screenshot
> was dlabel/diff/aparc+aseg.flt.**nii.gz.
>
> a.y
>
>
> On Wed, 27 Mar 2013, Susan Kuo wrote:
>
>  Hi Anastasia,
>>   I see what you mean. I had previously used the dtifit_FA.nii.gz image
>> and
>> overlaid that with the aparc+aseg+2mm.nii.gz image in /dlabel/diff, using
>> freeview. I didn't end up with the same images as you, however. Can you
>> tell
>> me which images you used to obtain this overlay?
>>
>>   I am going to run bbregister tonight, and I'll let you know how it goes
>> --
>> I'm hopeful!
>>
>> Thank you again!
>>
>> Susie Kuo
>> NIH
>>
>> On Tue, Mar 26, 2013 at 5:35 PM, Anastasia Yendiki
>>  wrote:
>>
>>   Hi Susie - I'm attaching a snapshot from your subject, showing
>>   the aparc+aseg overlaid on the FA map. The registration has
>>   failed. The frontal lobe has spilled out of the brain, the white
>>   matter has spilled into the ventricles.
>>
>>   I strongly recommend using bbregister for the intra-subject
>>   registration, which is the default in the latest version of
>>   trac-all.
>>
>>   Hope this helps,
>>   a.y
>>
>>   On Tue, 26 Mar 2013, Susan Kuo wrote:
>>
>> Hi Anastasia,   I did as you recommended and checked
>> the diffusion-to-anatomical
>> registration, and the overlay of aparc+aseg_mask on
>> FA, and these views seem to be
>> good. Upon closer inspection, what I find is that
>> there are incipient 'bits' of all
>> the tracts, but they seem to not have 'grown',
>> though they are in the proper space
>> (comparing them to good brains that yielded the full
>> complement of tracts). Is there a
>> configuration in your TRACULA that controls the
>> growing of the tracts specifically?
>> Perhaps I should look into that.
>>
>>   Thank you, btw, for your very prompt reply
>> yesterday- it was much appreciated!
>>
>>
>> Sincerely,
>> Susie Kuo
>> NIH
>>
>> On Mon, Mar 25, 2013 at 4:58 PM, Anastasia Yendiki
>> 
>> wrote:
>>
>>   Hi Susan - Good to hear that you get good
>> results for most of your
>>   subjects. Have you checked the aparc+aseg and
>> the diffusion-to-anatomical
>>   registration for the subjects that are
>> failing? I'd check the
>>   aparc+aseg_mask (in the dlabel/diff directory)
>> over the FA map to see if
>>   there are any holes or misregistration.
>>
>>   a.y
>>
>>   On Mon, 25 Mar 2013, Susan Kuo wrote:
>>
>> Hi FreeSurfers and Anastasia,   TRACULA
>> is working great for
>> me, generating tracts for a sample of 20
>> subject
>> brains I'm working with. However, for 3
>> of the brains, I'm
>> receiving incomplete and poorly formed
>> tracts.
>> I've re-run trac-all at least 2x on each
>> subject in case there
>> was a mistake in my original
>> configuration.
>> However, I am reproducing the same
>> results. Does anybody have
>> an idea why I would see these "spotty"
>> tracts?
>>
>>
>> Thank you for al

Re: [Freesurfer] Incomplete and poorly formed tracts

2013-03-27 Thread Susan Kuo
Hi Anastasia,
  Thank you! I can see that the aparc+aseg spilled over badly. I am going
to try bbregister, and then re-betting (Brain Extraction Tool-ing) the
original image, since the dtifit_FA looks to include non-brain near the
frontal lobe. I'll keep you and the FS community updated on the results, in
case anybody is curious.


Sincerely,
Susie Kuo
NIH


On Wed, Mar 27, 2013 at 10:41 AM, Anastasia Yendiki <
ayend...@nmr.mgh.harvard.edu> wrote:

>
> Hi Susie - The -tv (tract volume) option of freeview is designed
> specifically to display the merged*.mgz output files of tracula. For a
> regular volume, use -v or no option at all. For best displaying the
> aparc+aseg, select it in freeview and choose "Lookup Table" from the Color
> map menu.
>
> Hope this helps,
> a.y
>
> On Wed, 27 Mar 2013, Susan Kuo wrote:
>
>  Hi Anastasia,   I'm attaching the graphic I arrived at with the command:
>>
>> > freeview -tv /dlabel/diff/aparc+**aseg.flt.nii.gz -v
>> /dmri/dtifit_FA.**nii.gz
>>
>> Here, I used the subject I send you (Sa69845.5) for the subjectID. I
>> could not detect
>> spill over in these images, mostly because the frontal lobe is a little
>> cut off. Did
>> you perhaps use a different viewer? I'm hoping to be able to detect these
>> mis-registrations better for myself in the future. Thanks for all your
>> help!
>>
>> Sincerely,
>> Susie Kuo
>> NIH
>>
>> On Wed, Mar 27, 2013 at 1:32 AM, Anastasia Yendiki <
>> ayend...@nmr.mgh.harvard.edu>
>> wrote:
>>
>>   Hi Susie - Everything under dlabel/diff is mapped to DWI space
>> (either
>>   with flirt or with bbregister, whichever you use). The one in my
>>   screenshot was dlabel/diff/aparc+aseg.flt.**nii.gz.
>>
>>   a.y
>>
>>   On Wed, 27 Mar 2013, Susan Kuo wrote:
>>
>> Hi Anastasia,
>>   I see what you mean. I had previously used the
>> dtifit_FA.nii.gz image and
>> overlaid that with the aparc+aseg+2mm.nii.gz image in
>> /dlabel/diff, using
>> freeview. I didn't end up with the same images as you,
>> however. Can you tell
>> me which images you used to obtain this overlay?
>>
>>   I am going to run bbregister tonight, and I'll let you know
>> how it goes --
>> I'm hopeful!
>>
>> Thank you again!
>>
>> Susie Kuo
>> NIH
>>
>> On Tue, Mar 26, 2013 at 5:35 PM, Anastasia Yendiki
>>  wrote:
>>
>>   Hi Susie - I'm attaching a snapshot from your subject,
>> showing
>>   the aparc+aseg overlaid on the FA map. The registration
>> has
>>   failed. The frontal lobe has spilled out of the brain,
>> the white
>>   matter has spilled into the ventricles.
>>
>>   I strongly recommend using bbregister for the
>> intra-subject
>>   registration, which is the default in the latest version
>> of
>>   trac-all.
>>
>>   Hope this helps,
>>   a.y
>>
>>   On Tue, 26 Mar 2013, Susan Kuo wrote:
>>
>> Hi Anastasia,   I did as you recommended and
>> checked
>> the diffusion-to-anatomical
>> registration, and the overlay of aparc+aseg_mask
>> on
>> FA, and these views seem to be
>> good. Upon closer inspection, what I find is that
>> there are incipient 'bits' of all
>> the tracts, but they seem to not have 'grown',
>> though they are in the proper space
>> (comparing them to good brains that yielded the
>> full
>> complement of tracts). Is there a
>> configuration in your TRACULA that controls the
>> growing of the tracts specifically?
>> Perhaps I should look into that.
>>
>>   Thank you, btw, for your very prompt reply
>> yesterday- it was much appreciated!
>>
>>
>> Sincerely,
>>   

Re: [Freesurfer] Incomplete and poorly formed tracts

2013-03-27 Thread Susan Kuo
Hi Anastasia,
  I saved out some of the completed trac-all.cmds following each TRACULA
'step', and I see where aparc+aseg is mapped to diffusion space. Thank you
again for all your help - my project is starting to look a lot better!


Sincerely,
Susie Kuo
NIH

On Wed, Mar 27, 2013 at 12:02 PM, Anastasia Yendiki <
ayend...@nmr.mgh.harvard.edu> wrote:

>
> Actually the brain mask used on the diffusion data *is* the aparc+aseg
> mapped to diffusion space, so the reason you see that extra non-brain stuff
> in the FA map is because the aparc+aseg was not aligned well to it.
>
> So you just need to switch on bbregister and switch off flirt in your
> configuration file, and then rerun trac-all. If the DWI-to-T1 registration
> is good, then the masking issue will be solved too.
>
> Good luck!
>
> a.y
>
> On Wed, 27 Mar 2013, Susan Kuo wrote:
>
>  Hi Anastasia,   Thank you! I can see that the aparc+aseg spilled over
>> badly. I am
>> going to try bbregister, and then re-betting (Brain Extraction Tool-ing)
>> the original
>> image, since the dtifit_FA looks to include non-brain near the frontal
>> lobe. I'll keep
>> you and the FS community updated on the results, in case anybody is
>> curious.
>>
>>
>> Sincerely,
>> Susie Kuo
>> NIH
>>
>>
>> On Wed, Mar 27, 2013 at 10:41 AM, Anastasia Yendiki <
>> ayend...@nmr.mgh.harvard.edu>
>> wrote:
>>
>>   Hi Susie - The -tv (tract volume) option of freeview is designed
>>   specifically to display the merged*.mgz output files of tracula.
>> For a
>>   regular volume, use -v or no option at all. For best displaying the
>>   aparc+aseg, select it in freeview and choose "Lookup Table" from
>> the Color
>>   map menu.
>>
>>   Hope this helps,
>>   a.y
>>
>>   On Wed, 27 Mar 2013, Susan Kuo wrote:
>>
>> Hi Anastasia,   I'm attaching the graphic I arrived at with
>> the command:
>>
>> > freeview -tv /dlabel/diff/aparc+**
>> aseg.flt.nii.gz
>> -v
>> /dmri/dtifit_FA.**nii.gz
>>
>> Here, I used the subject I send you (Sa69845.5) for the
>> subjectID. I could not detect
>> spill over in these images, mostly because the frontal lobe is
>> a little cut off. Did
>> you perhaps use a different viewer? I'm hoping to be able to
>> detect these
>> mis-registrations better for myself in the future. Thanks for
>> all your help!
>>
>> Sincerely,
>> Susie Kuo
>> NIH
>>
>> On Wed, Mar 27, 2013 at 1:32 AM, Anastasia Yendiki
>> 
>> wrote:
>>
>>   Hi Susie - Everything under dlabel/diff is mapped to DWI
>> space (either
>>   with flirt or with bbregister, whichever you use). The
>> one in my
>>   screenshot was dlabel/diff/aparc+aseg.flt.**nii.gz.
>>
>>   a.y
>>
>>   On Wed, 27 Mar 2013, Susan Kuo wrote:
>>
>> Hi Anastasia,
>>   I see what you mean. I had previously used the
>> dtifit_FA.nii.gz image and
>> overlaid that with the aparc+aseg+2mm.nii.gz image
>> in
>> /dlabel/diff, using
>> freeview. I didn't end up with the same images as
>> you,
>> however. Can you tell
>> me which images you used to obtain this overlay?
>>
>>   I am going to run bbregister tonight, and I'll
>> let you know
>> how it goes --
>> I'm hopeful!
>>
>> Thank you again!
>>
>> Susie Kuo
>> NIH
>>
>> On Tue, Mar 26, 2013 at 5:35 PM, Anastasia Yendiki
>>  wrote:
>>
>>   Hi Susie - I'm attaching a snapshot from
>> your subject,
>> showing
>>   the aparc+aseg overlaid on the FA map. The
>> registration
>>         has
>>   fail

[Freesurfer] Use our own training subjects?

2013-04-15 Thread Susan Kuo
Hi Anastasia,

   We were viewing the arcuate fasiculus tract created by TRACULA, and
noticed that the tract did not reach Broca's area. While there exists
literature that now challenges this neuroanatomical convention, I would
like to investigate the possibility of creating and inputting on our own
training subjects as priors. Do you know if we can try this? Thank you!

-- 
Susie Kuo

Mediocrity knows nothing higher than itself, but talent instantly
recognizes genius. - Sir Arthur Conan Doyle,* Sherlock Holmes- Valley of
Fear*
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[Freesurfer] Which space is TRACULA statistics in?

2013-05-01 Thread Susan Kuo
Hi FreeSurfing researchers,
  I am looking at the pathstats.overall.txt, and wanted to see if anybody
knew which space these voxels are in - I assume MNI space (given that I
used the MNI template) and not individual diffusion space, but would like
to confirm. Does anybody know for certain what space this data is reported
in?


Thanks!

-- 
Susie Kuo
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Re: [Freesurfer] Which space is TRACULA statistics in?

2013-05-01 Thread Susan Kuo
Thank you, Anastasia! I will keep this in mind when I analyze the group
statistics.





On Wed, May 1, 2013 at 11:07 AM, Anastasia Yendiki <
ayend...@nmr.mgh.harvard.edu> wrote:

>
> Hi Susan - All outputs of tracula are in native diffusion space.
> Tractography is performed in the native space, without any transformationon
> the DWIs to another space.
>
> a.y
>
>
> On Wed, 1 May 2013, Susan Kuo wrote:
>
>  Hi FreeSurfing researchers,   I am looking at the pathstats.overall.txt,
>> and wanted to see if anybody knew
>> which space these voxels are in - I assume MNI space (given that I used
>> the MNI template) and not
>> individual diffusion space, but would like to confirm. Does anybody know
>> for certain what space this data
>> is reported in?
>>
>>
>> Thanks!
>>
>> --
>> Susie Kuo
>>
>>
>>
>>
>>
>
> The information in this e-mail is intended only for the person to whom it
> is
> addressed. If you believe this e-mail was sent to you in error and the
> e-mail
> contains patient information, please contact the Partners Compliance
> HelpLine at
> http://www.partners.org/**complianceline<http://www.partners.org/complianceline>.
>  If the e-mail was sent to you in error
> but does not contain patient information, please contact the sender and
> properly
> dispose of the e-mail.
>



-- 
Susie Kuo

Mediocrity knows nothing higher than itself, but talent instantly
recognizes genius. - Sir Arthur Conan Doyle,* Sherlock Holmes- Valley of
Fear*
___
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Re: [Freesurfer] Use our own training subjects?

2013-05-01 Thread Susan Kuo
Thank you, Anastasia. I think we can recruit some hard-working
neuroanatomists here to help us with the labeling. I believe I will give
this a shot for the IFO. Again, thanks for your support on this - I really
appreciate it!

Susie Kuo


On Wed, May 1, 2013 at 11:04 AM, Anastasia Yendiki <
ayend...@nmr.mgh.harvard.edu> wrote:

>
> Hi Susan - Currently the only way to read in the manually labeled
> streamlines from the training subjects is in .trk format, which is what
> trackvis writes out. If you prefer to use another program for manual
> labeling, then its output would have to be converted to .trk format. This
> format is described in the online documentation for trackvis.
>
> The priors are generated by trac-all on the fly, given the .trk files and
> aparc+aseg for each of the training subjects. So once the manual labeling
> is done, it's just about transforming these files to the template space and
> passing the list to trac-all. I'm happy to document this part. The hard
> work is the manual labeling!
>
> Hope this helps,
> a.y
>
>
> On Wed, 1 May 2013, Susan Kuo wrote:
>
>  Hi Anastasia,  I would like to add several tracts of interest to our
>> priors, e.g. the IFO and sections of
>> the corpus callosum. Would it be possible for you to document how your
>> group created your priors? Are we
>> constrained to trackvis as a program? I know this is a lot of
>> documentation, but I would be happy to help.
>> I'm located on the NIH campus, and can travel up to help with the
>> documentation if you'd like. It would be
>> a good learning process for me. Thank you!
>>
>>
>> Susan Kuo
>>
>>
>> On Fri, Apr 26, 2013 at 1:42 PM, Anastasia Yendiki <
>> ayend...@nmr.mgh.harvard.edu> wrote:
>>
>>   Hi Susie - If you can label the connection that you want in
>> trackvis consistently in a set of
>>   subjects, then yes, it'd be possible to create your own mini atlas.
>> You'd have to map the .trk
>>   files with the trackvis streamlines and the aparc+aseg's from all
>> the training subjects to
>>   either the MNI or the CVS template. Then there's a trainfile
>> variable that you can set in the
>>   configuration file that tells trac-all where to find the training
>> data. If you decide to go
>>   through with this, I can try to document it in more detail on the
>> wiki.
>>
>>   a.y
>>
>>   On Mon, 15 Apr 2013, Susan Kuo wrote:
>>
>> Hi Anastasia,
>>We were viewing the arcuate fasiculus tract created by
>> TRACULA, and noticed that
>> the tract did not reach
>> Broca's area. While there exists literature that now
>> challenges this
>> neuroanatomical convention, I would
>> like to investigate the possibility of creating and inputting
>> on our own training
>> subjects as priors. Do
>> you know if we can try this? Thank you!
>>
>> --
>> Susie Kuo
>>
>> Mediocrity knows nothing higher than itself, but talent
>> instantly recognizes
>> genius. - Sir Arthur Conan
>> Doyle, Sherlock Holmes- Valley of Fear
>>
>>
>>
>>
>> The information in this e-mail is intended only for the person to whom it
>> is
>> addressed. If you believe this e-mail was sent to you in error and the
>> e-mail
>> contains patient information, please contact the Partners Compliance
>> HelpLine at
>> http://www.partners.org/**complianceline<http://www.partners.org/complianceline>.
>>  If the e-mail was sent to you in error
>> but does not contain patient information, please contact the sender and
>> properly
>> dispose of the e-mail.
>>
>>
>>
>>
>> --
>> Susie Kuo
>>
>> Mediocrity knows nothing higher than itself, but talent instantly
>> recognizes genius. - Sir Arthur Conan
>> Doyle, Sherlock Holmes- Valley of Fear
>>
>>


-- 
Susie Kuo

Mediocrity knows nothing higher than itself, but talent instantly
recognizes genius. - Sir Arthur Conan Doyle,* Sherlock Holmes- Valley of
Fear*
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Re: [Freesurfer] any way to parallelize Tracula?

2013-05-17 Thread Susan Kuo
Hi Anastasia,

  I apologize for jumping in here, but I have run bedpostx parallelized on
a cluster. Are there files that we can modify to run the script in
parallel? I noticed the '-prior' step is particularly long and cumbersome.
Thank you!


Susie Kuo
NIH


On Fri, May 17, 2013 at 12:47 PM, Anastasia Yendiki <
ayend...@nmr.mgh.harvard.edu> wrote:

>
> Hi Matt - Have you ever ran any parallelized FSL program (like bedpostx)
> on your SGE cluster? Our scripts have built on the FSL scripts, adding
> support for our PBS cluster, but in theory the SGE functionality is still
> there, so it should be possible to make it work. Of course practice is
> often somewhat more complicated than theory...
>
> a.y
>
>
> On Thu, 16 May 2013, Hoptman, Matthew wrote:
>
>
>> Hi all,
>> I have access to a cluster computer.  Is there any way to parallelize
>> tracula
>> using SGE without having to make a unique dmrirc for each subject?  If I
>> could
>> pass subject numbers to the dmrirc that would be great, for example.
>>
>> Thanks,
>>
>> Matt
>>
>>
>>
>> Matthew J. Hoptman, PhD
>> Research Scientist V
>> Nathan S. Kline Institute for Psychiatric Research
>>
>> Research Associate Professor in Psychiatry
>> NYU School of Medicine
>>
>>
>>
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-- 
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Mediocrity knows nothing higher than itself, but talent instantly
recognizes genius. - Sir Arthur Conan Doyle,* Sherlock Holmes- Valley of
Fear*
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Re: [Freesurfer] any way to parallelize Tracula?

2013-05-17 Thread Susan Kuo
Hi Anastasia,

 I am operating on 5.1... I'm glad to know that there is such a huge
difference in versions; and that there's so much gain in upgrading. I will
look at the fsl_sub_mgh script, and I'll post back what I figure out (if
anything), if other people are interested. Otherwise, it's vox clamantis en
deserto...

Thank you!
Susie Kuo
NIH


On Fri, May 17, 2013 at 1:02 PM, Anastasia Yendiki <
ayend...@nmr.mgh.harvard.edu> wrote:

>
> Hi Susie - The underlying script doing the job submission is called
> fsl_sub_mgh (a modification of fsl_sub).
>
> BTW, the -prior step should be about 70% less long and cumbersome in
> 5.2/5.3 than it was in 5.1. Which version are you running?
>
> a.y
>
>
> On Fri, 17 May 2013, Susan Kuo wrote:
>
>  Hi Anastasia,
>>   I apologize for jumping in here, but I have run bedpostx parallelized
>> on a
>> cluster. Are there files that we can modify to run the script in
>> parallel? I
>> noticed the '-prior' step is particularly long and cumbersome. Thank you!
>>
>>
>> Susie Kuo
>> NIH
>>
>>
>> On Fri, May 17, 2013 at 12:47 PM, Anastasia Yendiki <
>> ayend...@nmr.mgh.harvard.edu>
>> wrote:
>>
>>   Hi Matt - Have you ever ran any parallelized FSL program (like
>>   bedpostx) on your SGE cluster? Our scripts have built on the FSL
>>   scripts, adding support for our PBS cluster, but in theory the SGE
>>   functionality is still there, so it should be possible to make it
>>   work. Of course practice is often somewhat more complicated than
>>   theory...
>>
>>   a.y
>>
>>   On Thu, 16 May 2013, Hoptman, Matthew wrote:
>>
>>
>>   Hi all,
>>   I have access to a cluster computer.  Is there any way to
>>   parallelize tracula
>>   using SGE without having to make a unique dmrirc for each
>>   subject?  If I could
>>   pass subject numbers to the dmrirc that would be great, for
>>   example.
>>
>>   Thanks,
>>
>>   Matt
>>
>>
>>
>>   Matthew J. Hoptman, PhD
>>   Research Scientist V
>>   Nathan S. Kline Institute for Psychiatric Research
>>
>>   Research Associate Professor in Psychiatry
>>   NYU School of Medicine
>>
>>
>>
>>   IMPORTANT NOTICE: This e-mail is meant only for the use of the
>>   intended recipient.
>>   It may contain confidential information which is legally
>>   privileged or otherwise
>>   protected by law. If you received this
>>   e-mail in error or from someone who was not authorized to send
>>   it to you, you are
>>   strictly prohibited from reviewing, using, disseminating,
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>>   the e-mail.
>>
>>   PLEASE NOTIFY US IMMEDIATELY OF THE ERROR BY RETURN E-MAIL AND
>>   DELETE THIS MESSAGE
>>   FROM YOUR SYSTEM. Thank you for your cooperation.
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>>
>>
>> __**__**
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>> _
>> Conserve Resources. Print only when necessary.
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[Freesurfer] Editing the endpoint ROIs, and re-running trac-all -path

2013-07-09 Thread Susan Kuo
Hi Anastasia,

  For one of my subjects, I am noticing that the right uncinate fasciculus
is very diffuse and more importantly, erroneously crosses from the frontal
to the temporal lobe. I looked at the original endpoint ROI generated by
trac-all -prior (rh.unc_AS_avg33_mni_bbr_end1.nii.gz) and the dilated
endpoint ROI in the temporal lobe (rh.unc_AS_avg33_mni_bbr_end1_dil.nii.gz)
and noticed that the endpoints appeared to cross over into the frontal lobe.

  Attached is a snapshot for your perusal. On the left side is a display of
the /dmri/dtifit_FA.nii with the
/dpath/rh.unc_AS_avg33_mni_bbr/path.pd.nii.gz tract superimposed
over. As you can see, the right unc tract crosses from the frontal to the
temporal lobe. On the right side is a display of the
/dmri/brain_anat_mni.nii.gz with the endpoints
/dlabel/mni/rh.unc_AS_avg33_mni_bbr_end2.nii.gz in green
and /dlabel/mni/rh.unc_AS_avg33_mni_bbr_end1.nii.gz in blue (the
red dots are control points). As you can see, the blue endpoints bleed over
from the temporal to the frontal lobe.

  I tried to eliminate these blue points with fslview and then writing over
rh.unc_AS_avg33_mni_bbr_end1.nii.gz. I performed a similar function
manually editing rh.unc_AS_avg33_mni_bbr_end1_dil.nii.gz to better reflect
the boundaries . I subsequently tried to re-run trac-all -path, and
 unfortunately, all my tracts looked much worse.


   Do you know if incorrectly placed endpoints impair the path
reconstruction? Do you think you can help me? Any advice you can give would
be much appreciated.








-- 
Susie Kuo
NIH

Mediocrity knows nothing higher than itself, but talent instantly
recognizes genius. - Sir Arthur Conan Doyle,* Sherlock Holmes- Valley of
Fear*
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[Freesurfer] pathstats.byvoxel.txt

2013-07-15 Thread Susan Kuo
Hi Anastasia,
   I noticed that my pathstats.byvoxel.txt files are missing the last four
values (AD_Avg RD_Avg MD_Avg FA_Avg)  even though I'm certain I ran
trac-all -path -c  correctly. Do you have any idea why this
might be? e.g. a configuration file change?

Thank you!

-- 
Susie Kuo

Mediocrity knows nothing higher than itself, but talent instantly
recognizes genius. - Sir Arthur Conan Doyle,* Sherlock Holmes- Valley of
Fear*
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[Freesurfer] pathstats.byvoxel.txt

2013-07-15 Thread Susan Kuo
Hi Anastasia,
  I believe the issue I had with the pathstats.byvoxel.txt not displaying
the last four values (AD_Avg RD_Avg MD_Avg FA_Avg
) is owing to the fact that trac-all was run with FREESURFER 5.1. I believe
that FREESURFER 5.2 may be needed to generate path.pd.trk, and from that,
the above values are derived. Is this correct? Thank you again, and have a
good day!
-- 
Susie Kuo

Mediocrity knows nothing higher than itself, but talent instantly
recognizes genius. - Sir Arthur Conan Doyle,* Sherlock Holmes- Valley of
Fear*
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[Freesurfer] Problems in testing out newly installed Freesurfer with autorecon-1 option

2013-01-09 Thread Susan Kuo
Hi,
  I'm currently in the process of verifying that my install of Freesurfer
works. I have an Ubuntu 12.04.1 LTS box. I encountered what appeared to be
4 errors after running  *recon-all -s bert -autorecon1.*
The last line I included here states that the command completed without
error, so I'm not sure if I should be concerned that*nu_correct* wasn't
found, or that there were loading errors, and a *mincREAD()* error. Could
you help me identify what I need to do to correct these issues? I've
included the standard output for this command as a text file here. There
appear to be 4 issues:


***ISSUE 1: cannot find *nu_correct* *
nu_correct: Command not found.
INFO: current FREESURFER_HOME does not match that of previous processing.

***ISSUE 2: No such file or directory error
cp: cannot stat `./tmp.mri_nu_correct.mni.25253/nu2.mnc': No such file or
directory
mri_binarize --i ./tmp.mri_nu_correct.mni.25253/nu2.mnc --min -1 --o
./tmp.mri_nu_correct.mni.25253/ones.mgz
mincRead(): can't find file ./tmp.mri_nu_correct.mni.25253/nu2.mnc

***ISSUE 3: Loading error**
Loading ./tmp.mri_nu_correct.mni.25253/ones.mgz
ERROR: loading ./tmp.mri_nu_correct.mni.25253/ones.mgz
mri_segstats --id 1 --seg ./tmp.mri_nu_correct.mni.25253/ones.mgz --i
./tmp.mri_nu_correct.mni.25253/nu2.mnc --sum
./tmp.mri_nu_correct.mni.25253/sum.junk --avgwf
./tmp.mri_nu_correct.mni.25253/output.mean.dat
mghRead(./tmp.mri_nu_correct.mni.25253/ones.mgz, -1): could not open file

***ISSUE 4: Loading error**
Loading ./tmp.mri_nu_correct.mni.25253/ones.mgz
ERROR: loading ./tmp.mri_nu_correct.mni.25253/ones.mgz
(standard_in) 1: syntax error
mris_calc -o ./tmp.mri_nu_correct.mni.25253/nu2.mnc
./tmp.mri_nu_correct.mni.25253/nu2.mnc mul
mincRead(): can't find file ./tmp.mri_nu_correct.mni.25253/nu2.mnc

This is the last line to standard output, and it seems
reassuring**
recon-all -s bert finished without error at Tue Jan  8 17:49:19 EST 2013


I appreciate whatever help you can give me, and thank you very much for
your time! The attached is a text file.


Best,
S Kuo


freesurfer
Description: Binary data
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[Freesurfer] Fwd: Problems in testing out newly installed Freesurfer with autorecon-1 option

2013-01-10 Thread Susan Kuo
Thank you, Nick. I tried using fixup_mni_paths, but received a poor result.
I copied the stdout below:

***
yocto/j/dti7/Susie/freesurfer/subjects> su
Password:
root@yocto:/j/dti7/Susie/freesurfer/subjects# fixup_mni_paths
fixup_mni_paths: command not found
root@yocto:/j/dti7/Susie/freesurfer/subjects# cd $FREESURFER_HOME/bin
root@yocto:/usr/local/freesurfer/bin# fixup_mni_paths
fixup_mni_paths: command not found
root@yocto:/usr/local/freesurfer/bin# ./fixup_mni_paths
Using perl '/usr/bin/perl'
Patching autocrop...(original file saved to autocrop.old)
Patching field2imp...(original file saved to field2imp.old)
Patching imp2field...(original file saved to imp2field.old)
Patching make_template...(original file saved to make_template.old)
Patching mritoself...(original file saved to mritoself.old)
Patching mritotal...(original file saved to mritotal.old)
Patching nu_correct...(original file saved to nu_correct.old)
Patching nu_estimate...(original file saved to nu_estimate.old)
Patching nu_estimate_np_and_em...(original file saved to
nu_estimate_np_and_em.old)
Patching nu_evaluate...(original file saved to nu_evaluate.old)
Patching resample_labels...(original file saved to resample_labels.old)
Patching sharpen_volume...(original file saved to sharpen_volume.old)
Patching xfmtool...(original file saved to xfmtool.old)
done.
Here are the changes:
diff autocrop autocrop.old
diff field2imp field2imp.old
diff imp2field imp2field.old
diff make_template make_template.old
diff mritoself mritoself.old
diff mritotal mritotal.old
diff nu_correct nu_correct.old
diff nu_estimate nu_estimate.old
diff nu_estimate_np_and_em nu_estimate_np_and_em.old
diff nu_evaluate nu_evaluate.old
diff resample_labels resample_labels.old
diff sharpen_volume sharpen_volume.old
diff xfmtool xfmtool.old
done.
Note: not all systems will require a patch,
and may not show any differences.
Now a test of the MNI tool 'nu_correct'...
nu_correct: Command not found.
A version number and usage message should have been displayed.
***

I double-checked that /usr/bin/perl exists, so that's ok. I've been
crawling the forums, but I can't find a good answer for what appears to be
a simple problem. Can I fix this manually?

Thanks!
Susie

On Wed, Jan 9, 2013 at 4:29 PM, Nick Schmansky wrote:

> Susan,
>
> there appear to be two problems here.  the first and main problem is the
> one:
>
> nu_correct: Command not found.
>
> nu_correct is a script which has as its first line:
>
> #!/usr/bin/perl -w
>
> so can you check that you have perl installed at /usr/bin/perl?
>
> better yet, run this script:
>
> fixup_mni_paths
>
> which does the checking for you and attempts to fix the scripts.
>
> the other problem appears to be the fact that mri_nu_correct.mni doesnt
> exit upon finding the error that the files are missing.  this is bad,
> although in looking at the script, it ought to.   the next release will
> contain a double check.  from the looks of your script, it appears that
> you are running recon-all with a subject that had its data already
> processed, so nu.mgz existed already, so it just used that and continued
> on.  so the results are correct (assuming nu.mgz was decent to begin
> with), but resolving the nu_correct issue is the important thing to do.
>
> Nick
>
>
> On Wed, 2013-01-09 at 11:00 -0500, Susan Kuo wrote:
> > Hi,
> >   I'm currently in the process of verifying that my install of
> > Freesurfer works. I have an Ubuntu 12.04.1 LTS box. I encountered what
> > appeared to be 4 errors after running  recon-all -s bert -autorecon1.
> > The last line I included here states that the command completed
> > without error, so I'm not sure if I should be concerned
> > thatnu_correct wasn't found, or that there were loading errors, and
> > a mincREAD() error. Could you help me identify what I need to do to
> > correct these issues? I've included the standard output for this
> > command as a text file here. There appear to be 4 issues:
> >
> >
> >
> >
> > ***ISSUE 1: cannot find nu_correct *
> > nu_correct: Command not found.
> > INFO: current FREESURFER_HOME does not match that of previous
> > processing.
> >
> >
> > ***ISSUE 2: No such file or directory error
> > cp: cannot stat `./tmp.mri_nu_correct.mni.25253/nu2.mnc': No such file
> > or directory
> > mri_binarize --i ./tmp.mri_nu_correct.mni.25253/nu2.mnc --min -1
> > --o ./tmp.mri_nu_correct.mni.25253/ones.mgz
> > mincRead(): can't find file ./tmp.mri_nu_correct.mni.25253/nu2.mnc
> >
> >
> > ***ISSUE 3: Loading error**
> > Loading ./tmp.mri_nu_correct.mni.25253/ones.mgz
> > ERROR: loadi

[Freesurfer] Problem with flip4fsl

2013-02-06 Thread Susan Kuo
Hi FreeSurfer community,
  I am trying to run 'trac-all -corr -c ' on the tutorial
data, specifically for the single subject: Diff001. To start from
'scratch', I've deleted all but the './scripts' and the './orig'
directories in the Diff001 parent folder. When I run the following command:

> trac-all -corr -c 

I receive the following error:

ERROR BEGINS>
flip4fsl
/j/dti7/Susie/freesurfer/subjects/diffusion_tutorial/Diff001/dmri/dwi_orig.nii.gz
/j/dti7/Susie/freesurfer/subjects/diffusion_tutorial/Diff001/dmri/dwi_orig_flip.nii.gz
INFO: input image orientation is LPS
INFO: input image determinant is 8
fslswapdim
/j/dti7/Susie/freesurfer/subjects/diffusion_tutorial/Diff001/dmri/dwi_orig.nii.gz
x -y z
/j/dti7/Susie/freesurfer/subjects/diffusion_tutorial/Diff001/dmri/dwi_orig_flip.nii.gz
fslorient -forceradiological
/j/dti7/Susie/freesurfer/subjects/diffusion_tutorial/Diff001/dmri/dwi_orig_flip.nii.gz
Cannot open volume
/j/dti7/Susie/freesurfer/subjects/diffusion_tutorial/Diff001/dmri/dwi_orig_flip
for reading!
> ERROR ENDS


Looking in my 'Diff001/dmri' directory, I cannot locate a
'dwi_orig_flip.nii.gz' file. I assume this is where the script breaks. I
tried to run the 'flip4fsl' command singly, but received the same error.

I'm working on a:
3.2.0-35-generic #55-Ubuntu SMP Wed Dec 5 17:42:16 UTC 2012 x86_64 x86_64
x86_64 GNU/Linux

I've unsuccessfully searched through the mailing list archive to find if
others had encountered the same issue previously. Does anybody have any
suggestions?


Thanks!
Susie
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[Freesurfer] Troubleshooting the preproc

2013-02-06 Thread Susan Kuo
Hi FreeSurfer community,

  I am currently trying to troubleshoot my preprocessing part of TRACULA
with the tutorial dataset (for *Diff001* as the subject, specifically),
running *trac-all -corr -c *. I am running the commands in *
subjectID/scripts/trac-all.cmd* one by one because I've had problems with
some of the steps. Specifically, *flip4fsl* didn't work. I instead
used *fslswapdim
-x -y z* (*flip4fsl* uses *fslswapdim x -y z*, which doesn't generate the
correct orientation for FSL in fact), followed by *fslorient
-forceradiological*. I successfully ran *eddy_current *thereafter. Now I am
stuck again, at FreeSurfer's *xfmrot, *which should look like:
*
*
*xfmrot   []*

I specifically typed in:

*xfmrot
/j/dti7/Susie/freesurfer/subjects/diffusion_tutorial/Diff001/dmri/dwi.ecclog
/j/dti7/Susie/freesurfer/subjects/diffusion_tutorial/Diff001/dmri/bvecs.norot
/j/dti7/Susie/freesurfer/subjects/diffusion_tutorial/Diff001/dmri/bvecs*


and received the following iterative errors:

*
avscale: Command not found.
R: Subscript out of range.
R: Subscript out of range.
R: Subscript out of range.
*

Can any of you help me?

Thank you,
Susie K
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[Freesurfer] Trying to email out on the FreeSurfer mailing list

2013-02-07 Thread Susan Kuo
Hi,
  I've been trying to email out for help to the FreeSurfer community, using
the below addresses. However, I am not receiving my own email back, though
I am a member of the mailing list via this email address. Can you tell me
what I'm doing wrong?

Thank you!
Susan Kuo

-- Forwarded message ------
From: Susan Kuo 
Date: Wed, Feb 6, 2013 at 4:57 PM
Subject: Troubleshooting the preproc
To: ayend...@nmr.mgh.harvard.edu
Cc: freesurfer@nmr.mgh.harvard.edu


Hi FreeSurfer community,

  I am currently trying to troubleshoot my preprocessing part of TRACULA
with the tutorial dataset (for *Diff001* as the subject, specifically),
running *trac-all -corr -c *. I am running the commands in *
subjectID/scripts/trac-all.cmd* one by one because I've had problems with
some of the steps. Specifically, *flip4fsl* didn't work. I instead
used *fslswapdim
-x -y z* (*flip4fsl* uses *fslswapdim x -y z*, which doesn't generate the
correct orientation for FSL in fact), followed by *fslorient
-forceradiological*. I successfully ran *eddy_current *thereafter. Now I am
stuck again, at FreeSurfer's *xfmrot, *which should look like:
*
*
*xfmrot   []*

I specifically typed in:

*xfmrot
/j/dti7/Susie/freesurfer/subjects/diffusion_tutorial/Diff001/dmri/dwi.ecclog
/j/dti7/Susie/freesurfer/subjects/diffusion_tutorial/Diff001/dmri/bvecs.norot
/j/dti7/Susie/freesurfer/subjects/diffusion_tutorial/Diff001/dmri/bvecs*


and received the following iterative errors:

*
avscale: Command not found.
R: Subscript out of range.
R: Subscript out of range.
R: Subscript out of range.
*

Can any of you help me?

Thank you,
Susie K
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[Freesurfer] BEDPOSTX - seychelles option

2013-02-07 Thread Susan Kuo
Hi FreeSurfer community,

  I would like to run '-bedp' with TRACULA, to run the bedpostx option. I
see from the *./subjID/scripts/trac-all.cmd* file that the actual command
issued was *bedpostx_seychelles*, which allows for the PBS queueing system
running on seychelles and is used by the Martinos Center folks to
parallelize computation. I have 2 questions regarding this modification:

1 - Can I switch to strictly *bedpostx*? Is there a text file I can alter
to reflect changes to the commands issued? I believe *trac-all.cmd* is
actually created at runtime, not prior.

2 - I work at NIH and have the Biowulf cluster to work with. Can I
parallelize bedpostx on Biowulf (which uses a  Altair's PBS Pro batching
system) with TRACULA's *trac-all -bedp* command, or any of *trac-all*?

Thank you for your attention!


Susie Kuo
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[Freesurfer] Parallelizing on a different kind of cluster

2013-02-12 Thread Susan Kuo
Hi TRACULA Experts,

  Is there a way of parallelizing BEDPOSTX on a cluster, not using the
bedpostx_seychelles option since I'm on a non-MGH cluster?

Thanks!
Susie Kuo
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Re: [Freesurfer] Parallelizing on a different kind of cluster

2013-02-12 Thread Susan Kuo
I see. So I will discriminate between what bedpostx requires from the more
inclusive bedpostx_seychelles script. Thank you for your help. I sincerely
appreciate it.

Best,
Susie Kuo

On Tue, Feb 12, 2013 at 1:56 PM, Anastasia Yendiki <
ayend...@nmr.mgh.harvard.edu> wrote:

>
> Everything that's in bedpostx is also in bedpostx_seychelles. I've just
> added to it. But if you want to run bedpostx directly on the command line
> instead of going through trac-all -bedp, you can do that, too.
>
>
> On Tue, 12 Feb 2013, Susie Kuo wrote:
>
>  Thank you very much for your response. Is there a way to use bedpostx
>> instead of bedpostx_seychelles?
>>
>> If not, no worries. I will look at your script and compose one of our own.
>>
>>
>> Best,
>> Susie Kuo
>>
>> On Feb 12, 2013, at 11:56 AM, Anastasia Yendiki wrote:
>>
>>
>>> Hi Susie - FSL's bedpostx script works for parallelization on the type
>>> of cluster they have at Oxford, and bedpostx_seychelles is a modified
>>> version of it that also works on our cluster. Other than that, support for
>>> all possible cluster setups at other centers is beyond the scope of our
>>> research, but perhaps the existing script is useful as a starting point for
>>> making it work on your cluster.
>>>
>>> Hope this helps,
>>> a.y
>>>
>>> On Tue, 12 Feb 2013, Susan Kuo wrote:
>>>
>>>  Hi TRACULA Experts,
>>>>   Is there a way of parallelizing BEDPOSTX on a cluster, not using the
>>>> bedpostx_seychelles option since I'm
>>>> on a non-MGH cluster?
>>>> Thanks!
>>>> Susie Kuo
>>>>
>>>>
>>>
>>> The information in this e-mail is intended only for the person to whom
>>> it is
>>> addressed. If you believe this e-mail was sent to you in error and the
>>> e-mail
>>> contains patient information, please contact the Partners Compliance
>>> HelpLine at
>>> http://www.partners.org/**complianceline<http://www.partners.org/complianceline>.
>>>  If the e-mail was sent to you in error
>>> but does not contain patient information, please contact the sender and
>>> properly
>>> dispose of the e-mail.
>>>
>>
>>
>>
>>
>>
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[Freesurfer] TRACULA trac-all -path giving 'Segmentation error (core dumped) error'

2013-02-25 Thread Susan Kuo
Hi FreeSurfer Community,
   I am trying to run TRACULA's last step, 'trac-all -path -c
', and am receiving the error:

Segmentation fault(core dumped)

Moreover, when I look to standard output (terminal), I noticed that the
initial 'dmri_paths' command is incomplete. The terminal shows that the
command includes the '--init' switch and several arguments (e.g. '/dlabel/diff/rh.atr_PP_avg33_mni_flt_cpts_5.txt'), the last of which is
terminated mid-pathname. Can anyone suggest a reason why this would be the
case?




Thank you,
Susie Kuo
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