Re: [ccp4bb] A crystallisation screen pH query.

[Bernhard Rupp]

I would not take that for granted…see the comments by Tom Peat. It is easy to 
measure with some 

coarse and the fine strips both the unbuffered cocktail and drop pH – sometimes 
knowing the drop pH can be 

relevant for the physiological state. Mixing unbuffered cocktail and stock 
buffer also works

to give an initial idea.

 

LGBR

 

From: CCP4 bulletin board  On Behalf Of Jon Cooper
Sent: Sunday, October 6, 2019 00:47
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] A crystallisation screen pH query.

 

Many thanks for the excellent replies. I am very happy with the value of 5.1 
which two of you kindly sent.

 

Best wishes

 

Jon Cooper

 

On 5 Oct 2019 22:51, Bob Cudney mailto:b...@hrmail.com> > 
wrote:

Hello Jon,

 

That JCSG+ reagent is a copy of Hampton Research PEG/Ion reagent 48.  While 
there is no pH adjustment made during the formulation of this reagent, the 20% 
w/v PEG 3350, 0.2 M Ammonium citrate dibasic in deionized water results in a 
measured pH at 25 degrees Celsius of 5.1.  The formulation as well as measured 
pH, refractive index, conductivity and other details can be found here 
https://hamptonresearch.com/product-PEG-Ion-PEG-Ion-2-PEG-Ion-HT-11.html under 
the Support Material(s).  Hope this helps.

 

Kind Regards, Bob Cudney

 

From: CCP4 bulletin board mailto:CCP4BB@JISCMAIL.AC.UK> 
> On Behalf Of Jon Cooper
Sent: Saturday, October 5, 2019 2:10 PM
To: CCP4BB@JISCMAIL.AC.UK  
Subject: [ccp4bb] A crystallisation screen pH query.

 

Does anyone know the pH of JCSG+ condition A3 which is stated as 0.2 M ammonium 
citrate dibasic, 20% (v/v) PEG3350. I can't really measure it myself, so any 
help much appreciated!

 

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[ccp4bb] PhD and postdoc position - Structural Biology Methods development at Wuerzburg University

[Andrea Thorn]

Dear colleagues,

for two projects in structural biology methods development, we are
looking for a

PHD CANDIDATE (m/f/d)

and a

POSTDOCTORAL RESEARCHER (m/f/d)

The Thorn Laboratory the Rudolf Virchow Center at the University of
Wuerzburg  develops new methods for macromolecular X-ray crystallography
and Cryo-EM. In these exciting projects you will improve how well we
understand, measure and model macromolecular crystal structures using
practical experiments and big data analyses. We are looking for
candidates with skills in data analysis and programming. Any previous
experiences in crystallography, molecular biology, machine learning,
image or statistical analysis are also a bonus. You should be a good
communicator. For the PhD project, we offer a 36-month full- or
part-time position, embedded in the Würzburg Graduate School for Life
Sciences (GSLS); for the postdoctoral position, we offer a 30-month
full- or part-time position with a competitive salary as well as career
and family support. Women are particularly encouraged to apply.

For more details, see:
https://www.uni-wuerzburg.de/fileadmin/4302/2019/Stellenanzeige_PhD_Thorn_September_2019_final_Auslage.pdf
https://www.uni-wuerzburg.de/fileadmin/4302/2019/Stellenanzeige_PostdocThorn_Sep_2019_final_Auslage.pdf

If you have any questions, please email me: andrea.th...@uni-wuerzburg.de.

Best wishes,



Andrea Thorn.

_

Dr. Andrea Thorn
group leader
Rudolf Virchow Center for Experimental Biomedicine
University of Wuerzburg
Josef-Schneider-Str. 2
97080 Wuerzburg
Germany

andrea.th...@uni-wuerzburg.de
https://www.uni-wuerzburg.de/en/rvz/research/associated-research-groups/thorn-group/



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