I'm not sure this is the right venue for your question (the mailing list is for package *development*). You might try opening an issue at the package bug-report site:

https://github.com/Bioconductor/Biostrings/issues

or asking on https://www.biostars.org/ forum (where there are a lot of bioinformatics people)

You may be asked to provide a reproducible example (e.g. by simulation or random generation ...)

  cheers
   Ben Bolker

On 6/16/21 9:14 PM, csmatyi wrote:
Hello,

I am trying to align two sequences, in DNAString format using the R command 
pairwiseAlignment.
I keep getting this error:

pairwiseAlignment(si,sj)
Error in .Call2("XStringSet_align_pairwiseAlignment", pattern, subject,  :
   max(nchar(pattern) * nchar(subject)) is too big (must be <= 2147483647)

The 2 DNAstrings are si and sj, and are 108,007 bp and 120,698 bp long, 
respectively.

Can someone help me? Are the sequences too long perchance?

Thanks, Matthew

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