Hi Everybody,

Now I have a doubt that is more statistical than R's technical. I’m working 
with ecology of recent Foraminifera.
At the lab we used to perform cluster analysis using 1-Pearson’s R and Wards 
method (we already saw it in bibliography of the area) which renders good 
results with our biological data. Recently, using “R” Software (vegan and 
Cluster packages) which allows the combination of any kind of distances matrix 
with any clustering method, we tried to used Bray Curtis + Wards (which seem to 
be more appropriate to a matrix with a lot of zeros) and it renders a better 
result. Furthermore, the results agree with our hypothesis and with the results 
we have got with the Distance-based Redundancy Analysis - dbRDA or CAP. It 
means, the analysis (Q-mode) clusters the stations according to the main 
physical, sedimentary and biological characteristics of the study area.

We received some critical comments noticing that Wards Method accepts Euclidean 
Distance only. So, we made the analysis again using Euclidean Distance but we 
don’t get the better results we had using 1-Pearson’s R + Wards or Bray Curtis 
+ Wards (actually any other distance + method combination rendered better 
results). Trying to find answers in the specialized literature we just got 
little more confused because in any moment we saw something like "You must use 
it with Euclidean Distance" and like I said above we already saw in some 
articles from respected journals, other kind of distance associated with the 
Ward's Clustering method. 

Is it wrong or is it “non sense” to do the analysis in the way we were doing?

The results with Wards combined with 1-Pearson’s R or Bray Curtis fit better 
with our hypothesis and have excellent agglomerative coefficients , but we 
don’t want to make inappropriate statistical procedures. I'm starting to 
realize how powerful R is, but it doesn't justify doing nonsense statistics...  
I hope one of you may help us!

Thank you in advance.

Rodrigo.

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