Is there possible to generate a barplot in the following link using ggplot?
https://photos.app.goo.gl/E3MC461dKaTZfHza9 here is what I did library(ggplot2) df <- read.csv(text= "trt,gene,freq,cols M6,ALDH16A1,100.0000000,red M6,Others,0.0000000,lightgrey M12,ALDH16A1,64.6638015,red M12,GBE1,2.0074865,#4C00FF M12,ZNF598,1.5832525,#004CFF M12,CHMP6,1.3503397,#00E5FF M12,C20orf27,1.2033828,#00FF4D M12,NEGR1,0.9676972,#4DFF00 M12,TNFAIP6,0.9122418,#E6FF00 M12,ZSCAN25,0.7375572,#FFFF00 M12,BCL2,0.6848745,#FFDE59 M12,CBL,0.6765562,#FFE0B3 M12,Others,25.2128102,lightgrey M18,ALDH16A1,42.4503581,red M18,ATF2,2.2360682,#4C00FF M18,DIAPH1,1.5256507,#004CFF M18,SESTD1,1.2053805,#00E5FF M18,TFCP2,1.1587958,#00FF4D M18,SCAPER,1.1180341,#4DFF00 M18,CUX1,1.0306877,#E6FF00 M18,TEX10,0.9841030,#FFFF00 M18,C6orf89,0.9666337,#FFDE59 M18,PTTG1IP,0.9258720,#FFE0B3 M18,Others,46.3984161,lightgrey") df$trt <- factor(df$trt,levels=unique(as.character(df$trt))) df$gene <- factor(df$gene,levels = unique(as.character(df$gene))) ggplot(df, aes(x=trt,y=freq, fill = gene))+geom_bar(stat = "identity", width = 0.5,color="black") + theme(axis.text.x = element_text(angle = 45, hjust = 1,size = 4)) df$cols is the color I want to use to label different gene in M6, M12,M18 as shown in Figure, and in each bar, the 'Others' of df$gene is always in the bottom of bar in M6,M12,M18 Thank you Aimin [[alternative HTML version deleted]] ______________________________________________ R-help@r-project.org mailing list -- To UNSUBSCRIBE and more, see https://stat.ethz.ch/mailman/listinfo/r-help PLEASE do read the posting guide http://www.R-project.org/posting-guide.html and provide commented, minimal, self-contained, reproducible code.