Hi Ana Marija,
It would help if we had the data, but I suspect that the data that we
don't have is not uniformly distributed:
library(plotrix)
ams_norm<-rnorm(100)
png("ams_norm.png")
qqunif(rescale(ams_norm,c(0,1)))
dev.off()
ams_unif<-runif(100)
png("ams_unif.png")
qqunif(ams_unif)
dev.off()
Jim
On Thu, Aug 15, 2019 at 3:27 AM Ana Marija <sokovic.anamar...@gmail.com> wrote:
>
> Hello,
>
> I was plotting QQ plot (in attach) via:
>
> qqunif = function(p, BH=T, MAIN = " ", SUB=" ")
> {
> nn = length(p)
> xx = -log10((1:nn)/(nn+1))
> plot( xx, -sort(log10(p)),
> main = MAIN, sub= SUB, cex.sub=1.3,
> xlab=expression(Expected~~-log[10](italic(p))),
> ylab=expression(Observed~~-log[10](italic(p))),
> cex.lab=1.0,mgp=c(2,1,0))
> abline(0,1,col='red')
> if(BH) ## BH = include Benjamini Hochberg FDR
> {
>
> abline(-log10(0.05),1, col='black',lty=1)
> text(0.5,1.9 , "FDR=0.05", col = "gray60",srt=45, cex=1)
> abline(-log10(0.10),1, col='black',lty=1)
> text(0.5, 1.6, "FDR=0.10", col = "gray60",srt=45, cex=1)
> abline(-log10(0.25),1, col='black',lty=1)
> text(0.5, 1.2, "FDR=0.25", col = "gray60",srt=45, cex=1)
> #legend('topleft', c("FDR = 0.05","FDR = 0.10","FDR = 0.25"),
> #col=c('black','black','black'),lty=c(1,1,1), cex=0.8)
> if (BF)
> {
> abline(h=-log10(0.05/nn), col='black') ## bonferroni
> }
> }
> }
>
> db=fread("/Users/ams/Desktop/GWAS_all.txt", header=T)
> dd=db[db$P<1e-3,]
> qqunif(dd$P)
>
> what do I need to change in my code so that FDR lines look like on the
> 2nd attachment?
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