Apologies for the phrasing of the question. I've sorted the problem (thanks Bert Gunter) by using the curly brackets {} as below (using a simplified version of my real model). I hope this helps someone else!
Jeff ----------------------- > data Alpha ip X 1 0.7106967 0.3616727 0.006027879 2 2.1678517 5.0615917 0.084359861 3 4.4066250 11.2282945 0.187138242 4 9.8495694 18.0534974 0.300891624 5 27.7247098 29.2064434 0.486774057 6 70.6931430 35.3946092 0.589910153 7 133.1240255 46.0347288 0.767245480 8 214.7851844 49.3811149 0.823018582 9 359.5511036 58.5069583 0.975115972 10 748.1840127 57.3744477 0.956240795 11 2129.9844080 60.0000000 1.000000000 > c<-1.83e-9 > cFe=c > model<-nls({Fe1<-cFe/(Alpha+1+k*c) + X~Alpha/(Alpha+1+k*c/(1+k*Fe1))},start=list(k=1e10)) > summary(model) Formula: X ~ Alpha/(Alpha + 1 + k * c/(1 + k * Fe1)) Parameters: Estimate Std. Error t value Pr(>|t|) k 3.491e+10 7.190e+09 4.856 0.000665 *** --- Signif. codes: 0 ‘***’ 0.001 ‘**’ 0.01 ‘*’ 0.05 ‘.’ 0.1 ‘ ’ 1 Residual standard error: 0.05589 on 10 degrees of freedom Number of iterations to convergence: 8 Achieved convergence tolerance: 2.393e-06 -- View this message in context: http://r.789695.n4.nabble.com/Non-linear-regression-with-complex-equation-tp4425942p4427617.html Sent from the R help mailing list archive at Nabble.com. ______________________________________________ R-help@r-project.org mailing list https://stat.ethz.ch/mailman/listinfo/r-help PLEASE do read the posting guide http://www.R-project.org/posting-guide.html and provide commented, minimal, self-contained, reproducible code.