On Feb 20, 2011, at 1:27 PM, Ben Ward wrote:

However, the

Y ~ X + Y^2

Produces the best fitting line - it is pretty much on the data points - I'm trying to make a standard curve, with which to take readings from a spectrophotometer off of. Rather than what I would normally use models for - such as hypothesis testing and analysis of data from experiments.

I thought we were leaving behind the model that had the dependent variable on both sides of hte equation. Can you explain how you would construct a chart or a function that will turn those results into something useful?


Thanks,
Ben.

On 20/02/2011 11:53, nzcoops wrote:
model<- lm(Approximate.Counts~X..Light.Transmission +
I(Approximate.Counts^2), data=Standards)

Might not be addressing the problem, don't you have Y ~ X + Y^2 here? That's
a violation of the assumptions of an lm isn't it?

Also for plotting CI on a curve look into ggplot2::geom_ribbon, it's much nicer than just plotting lines and is easy to use. had.co.nz should set you
right for setting this up.



David Winsemius, MD
West Hartford, CT

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