Hi Jim I am trying to prepare a bed file to load as accustom track on the UCSC genome browser. I have a data frame that looks like the one below. > x V1 V2 V3 1 chr1 11255 55 2 chr1 11320 29 3 chr1 11400 45 4 chr2 21680 35 5 chr2 21750 84 6 chr2 21820 29 7 chr2 31890 46 8 chr3 32100 29 9 chr3 52380 29 10 chr3 66450 46 I would like to insert the following 4 lines at the beginning: browser position chr1:1-10000 browser hide all track type=wiggle_0 name=sample description=chr1_sample visibility=full variableStep chrom=chr1 span=1 and then insert 2 lines before each chromosome: track type=wiggle_0 name=sample description=chr2_sample visibility=full vriableStep chrom=chr2 span=1 The final result should be tab delimited file that looks like this: browser position chr1:1-10000 browser hide all track type=wiggle_0 name=sample description=chr1_sample visibility=full variableStep chrom=chr1 span=1 chr1 11255 55 chr1 11320 29 chr1 11400 45 track type=wiggle_0 name=sample description=chr2_sample visibility=full variableStep chrom=chr2 span=1 chr2 21680 35 chr2 21750 84 chr2 21820 29 track type=wiggle_0 name=sample description=chr3_sample visibility=full variableStep chrom=chr3 span=1 chr3 32100 29 chr3 32170 45 chr3 32240 45 Any kind of help or guidance will be much appreciated. Joseph
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