I hope this can help: http://wxwidgets.org/manuals/2.6.1/wx_wxtreectrl.html#wxtreectrlsetitemfont http://wxwidgets.org/manuals/2.6.1/wx_wxtreectrl.html#wxtreectrlsetitembold
This pages are from the wxwidgets api reference but the functions are the same in wxPython Bye Ale -- http://mail.python.org/mailman/listinfo/python-list