Iain King wrote:
print >>nucleotides, seq[-76]last_part = line.rstrip()[-76 : ]You all mean: seq[:-76] , right? (assuming you've already stripped any junk off the end of the string)
The OP said "cut out the last 76 letters (nucleotides) from eachindividual sequence and send them to a new txt file with a similar format.", ie extract the last 76 letters (in the same format) to a file.
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