Hi All,

I’m trying to make an isomesh of EMD-6413 (a cryo map) in Pymol. It’s a large 
map with a lot of extra, not very clean. In Chimera I can just gaussian the map 
with a new map to the level I want with a slider and it looks good, but I’d 
much rather work in PyMOL!

PyMOL can gaussian maps, I do it all the time, but on maps that I create in 
PyMOL. How do I get an isomesh on EMD-6413 that I can gaussian?

Normally I would use:
alter all, q=1
set gaussian_resolution, 5
map_new mapA, gaussian, 1, pdb code, 6
isosurface surfA, mapA

But in in this case I don’t want to use a pdb, just the cryo map.

I can isosurface the cryo map with the following:
isosurface mapname, EMD-6413, 4 (any number between 1-6)

but that doesn’t allow me to apply the gaussian to the map…

How do I create an isosurface with a gaussian?

Thanks for any help,

H. Adam Steinberg
7904 Bowman Rd
Lodi, WI 53555
608/592-2366


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