Hi Christian,

Thanks so much for your email. I am looking forward to testing your approach 
and scripts soon. Again, thanks for the detailed explanations and the scripts. 

Best regards,
Marcelo

On Aug 25, 2014, at 6:02 PM, Christian Becke <christian.be...@fu-berlin.de> 
wrote:

> Hi,
> 
> Am 25.08.2014 21:44, schrieb Marcelo Marcet:
>> Thank you for taking the time to reply to this question and for
>> offering your help. I am also interested in quad-buffered stereo play
>> back.
> 
> Sorry, maybe I wasn't clear in my first mail: I don't have experience
> with quad-buffered stereo playback. All I have done is preparing 3D
> stereo movie files using PyMOL and ffmpeg[1] which I can play on a 3D TV 
> (using a side-by-side stereo movie file and polarization glasses) or on a 
> regular beamer (anaglyph red/cyan movie with red/cyan glasses).
> I expect that one could play these (or similarly prepared) movie files with a 
> movie player that makes use of OpenGL quad-buffered 3D capabilities of a 
> graphics card - but I never tried this myself.
> AFAIU, there is no such thing as a quad-buffered movie file - it's just the 
> movie player that plays back a 3D stereo movie file using OpenGL 
> quad-buffered stereo on hardware that supports it. A player that might work 
> is bino[2], but, as I said, I never got around to try it out.
> 
>> Would you be able to provide us with a bit more methodology information?
>> It sounds like you have a script that helps you save the side-by-side
>> images and later you use a software called ffmpeg to render the movie.
>> Is this correct?
> 
> Yes, this is correct. Here is how I did it:
> 1) Prepare a movie in PyMOL
> 2) run the attached python script ("run /path/to/mpng_3d.sh"). This will add 
> a new command to pymol: mpng_3d
> It works similar to the mpng command, but saves 2 images for each movie 
> frame, one for the left and one for the right eye. If ray traced frames are 
> desired, do "set ray_trace_frames, 1". You can also play around with the 
> stereo_angle setting (e.g. "set stereo_angle, 3"). This defines the 
> difference in viewing angle of the images for the left and right eye.
> The mpng_3d command takes the following options:
> mpng_3d <prefix>, <width>, <height>, [start=1], [end=-1]
> render stereoscopic frames sized <width> x <height> pixels.
> Files will be named <prefix>_%04d.png.
> Render frames <start> to <end> (default: all frames)
> 
> Example: "mpng_3d my_movie, 1920, 1080" will write png files called 
> my_movie_0001.png, my_movie_0002.png, ...
> If ray_trace_frames is set (recommended), the images will be ray traced and 
> have a size of 1920x1080 pixels (i.e. full HD).
> 3) Use ffmpeg to encode a movie from the individual frames saved with mpng_3d:
> For h.264 encoding:
> ffmpeg -i "my_movie_%04d.png" \
>       -an \
>       -r 30 -aspect 1.78 -pix_fmt yuv420p \
>       -c:v libx264 -tune animation \
>       -vf stereo3d=al:sbsl \
>       -profile:v baseline -level 3.0 -refs 4 -qmin 4 \
>       "my_movie.mp4"
> 
> For WMV encoding:
> ffmpeg -i "my_movie_%04d.png" \
>       -an \
>       -r 30 -aspect ${aspect} \
>        -vf stereo3d=al:sbsl \
>       -q:v 2 -c:v msmpeg4v3 \
>       "my_movie.wmv"
> 
> These are the commands I used on linux. Similar ffmpeg commands should also 
> work on Windows or OS X. Video quality and file size were reasonable with the 
> above settings, and the files played all right on almost all video players I 
> tested (the wmv files work with all versions of PowerPoint I tested, the 
> h.264 movies do not work with WinXP).
> If you change the size of the images, also change the -aspect parameter in 
> the ffmpeg commands accordingly (for 1920x1080 pixel images: 
> aspect=1920/1080=1.78).
> The above commands produce side-by-side stereo movies, i.e. the images for 
> the left and right eye are shown next to each other in each frame
> of the movie. Check the ffmpeg docs[3] for other output options.
> For some movie players (e.g. the one on my LG 3D TV) it might be necessary to 
> scale the pymol-rendered frames to half-width before encoding them to a 
> side-by-side stereo movie with ffmpeg. This can be done e.g. with the 
> "convert" command from the imagmagick[4] suite.
> 
> I hope this helps!
> 
> Christian
> 
> [1] http://ffmpeg.org
> [2] http://bino3d.org
> [3] http://ffmpeg.org/ffmpeg-filters.html#stereo3d
> [4] http://www.imagemagick.org
> 
> -- 
> Christian Becke
> 
> Freie Universität Berlin
> Fachbereich Biologie, Chemie, Pharmazie
> Institut für Chemie und Biochemie
> AG Strukturbiochemie
> 
> Takustr. 6
> 14195 Berlin
> Germany
> 
> Phone: +49 (0)30 838-57344
> Fax: +49 (0)30 838-56981
> E-mail: christian.be...@fu-berlin.de
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