Hi Sangita,

This is trickier than it should be in PyMOL.  The problem lies in the
fact that when you cut one residue it makes two chemically separate
entities that are stored internally as one object--so PyMOL tries to
re-connect the bonds.  One trick, that sometimes works is:

set connect_mode, 2
set connect_cutoff, 0

then load your file.

Cheers,

-- Jason

On Mon, Jun 7, 2010 at 12:24 PM, Sangita Kachhap <[email protected]> wrote:
>
>
> Hello all
>
> I have to cut phosphodiester bond in DNA.I am able to cut it by selecting pk1
> and pk2 and then deleting the bond. But I am not able to save the coordinate.
> When I am saving by "save molecule" and aging loading the saved molecule
> original molecule(Uncut DNA) is loaded.
>
> Can anybody suggest me how to save coordinate for cut DNA.
>
> With regard
> Sangita Kachhap
> JRF
> BIC,IMTECH
> CHANDIGARH
>
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-- 
Jason Vertrees, PhD
PyMOL Product Manager
Schrodinger, LLC

(e) [email protected]
(o) +1 (603) 374-7120

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