One of my tricks is to only show part of the structure at a time, ray
each part individually and the reassemble them in Photoshop. I do this
all the time with the huge multi protein PyMol sessions I have to work
on. It depends on your orientation in the viewer though, be careful to
not cut anything off. With the ribo you can hide the large or the small
subunit, render each separately, and then reassemble.
Michael Weber wrote:
Hi,
I have a problem when trying to ray 750,750 a quite large ribosome file
with maximum quality. Size of the relevant PyMOL v1.1 file is 100 MB (or
31 MB after reducing objects I do not need for the figure production).
PyMOL simply crashes under Windows Vista Home Premium as well as under
Ubuntu Linux v8.04 LTS without error report. My dual OS system is a
Samsung Aura Seven Notebook (Core2Duo @2.4 GHz each, 3 GB of RAM, NVIDIA
GeForce 9200M GS graphics board with 256 MB of RAM, 12" TFT @1280x800
resolution settings). I first thought the RAM under Vista is still
insufficient (Vista alone takes 900 MB of the 3 GB of memory) but then
I realized that this crash occurs as well under Linux. However, since
both OS are 32 bit and therefore to my (limited) knowledge cannot handle
more than 2 GB in a single task, RAM limitation might still be an issue
to think about, right?
I have experienced (and occasionally reported) this type of serious
crashes with PyMOL on a broad variety of machines over some years now -
usually occurring when trying to render final files - but, sadly, none
of these has ever been diagnosed and fixed properly. Maybe this time I
am lucky and someone knows what to do?
Best regards,
Michael.
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_______________________________________
H. Adam Steinberg
Artist, Scientist
<http://adam.steinbergs.us>
Information Technology and Media Center
Department of Biochemistry
University of Wisconsin-Madison
433 Babcock Drive
Madison, WI 53706
608/265-4982
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