Dear Sir,

      * I want too assign the secondary structure of my protein according
to DSSP classification,  I used the following commands*
                    *  alter A/22:28/,  ss='S'*
                           * or*
                     *alter A/45:50?, ss= 'H' *
                          *and so on  followed by *
                     *      rebuild*
*                                   cartoon automatic*
*                                   show cartoon*
*           But the problem is that the structure  remains the same, without
any alteration.*
*           I tried the same commands in case of other proteins, where it
works, but it failed in case of my protein. The protein is a globular
one      with  70  rsidues, having a crystal structure.  It has a single
chain only, so I used     alter /22:28/, ss= 'S' , I am interested to know
what type of  classification does Pymol follow for secondary structure
classification ,  and  where the problem lies so that I can not alter my
structure. Thanks in advance.*
**
**
*regards*
*SANGEETA     *

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