Dear Sir,
* I want too assign the secondary structure of my protein according to DSSP classification, I used the following commands* * alter A/22:28/, ss='S'* * or* *alter A/45:50?, ss= 'H' * *and so on followed by * * rebuild* * cartoon automatic* * show cartoon* * But the problem is that the structure remains the same, without any alteration.* * I tried the same commands in case of other proteins, where it works, but it failed in case of my protein. The protein is a globular one with 70 rsidues, having a crystal structure. It has a single chain only, so I used alter /22:28/, ss= 'S' , I am interested to know what type of classification does Pymol follow for secondary structure classification , and where the problem lies so that I can not alter my structure. Thanks in advance.* ** ** *regards* *SANGEETA *