Adi,
I have forwarded you some previous discussions on mutations. You will find
your answer below. If you need anything further, please let me know.
Best of Luck!
Cheers,
Nick
---------------------------------------------------
Hi,
I would like to ask how to change (modify or replace)
the amino acid residue? I'm using MacPymol and my
computer is iBookG4.
Thank you very much
Adi
----------------------------------------------------
----- Original Message -----
From: "Andrew Colasanti" <acolasa...@gmail.com>
To: <pymol-users@lists.sourceforge.net>
Sent: Thursday, April 20, 2006 9:36 AM
Subject: Re: [PyMOL] mutations
I follow the method outlined by Nick with one additional step, after
selecting the new residue you can run through a sidechain rotamer
library using the forward arrows at the bottom of the window. You
also have a choice to use backbone dependant or backbone independant
rotamers.
Andrew
On 4/18/06, Noinaj <noi...@uky.edu> wrote:
Orla,
This is simply, for the most part. Here is what I do.
Load your *.pdb file. Then under WIZARD in the menu bar, select
MUTAGENESIS. Then pretty much, follow directions. In the PyMOL VIEWER
window, it will ask you to ' pick a residue.' Once you pick a residue,
select the type of residue you want to mutate to by left clicking on [No
Mutation] button and holding down (under Mutagenesis). This brings up a
list of residues that you can then select from. Once you select the
residue
you want, hit APPLY, then DONE. If everything was done correctly, you
should see your mutated residue.
There are probably other ways to do this, but I hope this helps. If you
would like screenshots of the steps, just let me know.
Cheers,
Nick
----- Original Message -----
From: Orla O'Sullivan
To: pymol-users@lists.sourceforge.net
Sent: Tuesday, April 18, 2006 11:31 AM
Subject: [PyMOL] mutations
Hi all
I'm a newbie to Pymol and so far have been very impressed. However I have
tried to mutate some residues in my structure and have hit a brick wall.
Has anyone done this with pymol and let me know how to do it?
Regards
Orla
Dr.Orla O'Sullivan
Research Officer
Biotechnology
Moorepark Food Research Centre
Teagasc
Moorepark
Fermoy, Co. Cork
Ireland
Tel: +353 - (0)25 - 42344
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