Dear MARMAM readers,
I am pleased to announce the publication of the following article in Clinical and Vaccine Immunology, (abstract below): Hernández-Mora G, Manire C. A, González-Barrientos R., Barquero-Calvo E., Guzmán-Verri C., Staggs L., Thompson R., Chaves-Olarte E.,* and E. Moreno. *Serological Diagnosis of *Brucella *Infections in Odontocetes. 2009. Clin. Vaccine Immunol. Jun 906–915 *Abstract:* * * *Brucella ceti *causes disease in Odontoceti. The absence of control serum collections and the diversity of cetaceans have hampered the standardization of serological tests for the diagnosis of cetacean brucellosis. Without a “gold” standard for sensitivity and specificity determination, an alternative approach was followed. We designed an indirect enzyme-linked immunosorbent assay (iELISA) that recognizes immunoglobulins G (IgGs) from 17 odontocete species as a single group. For the standardization, we used *Brucella melitensis *and *Brucella abortus *lipopolysaccharides, serum samples from seven resident odontocetes with no history of infectious disease displaying negative rose bengal test (RBT) reactions, and serum samples from seven dolphins infected with *B. ceti. *We compared the performance of the iELISA with those of the protein G ELISA (gELISA), the competitive ELISA (cELISA), and the immunofluorescence (IF) and dot blot (DB) tests, using 179 odontocete serum samples and RBT as the reference. The diagnostic potential based on sensitivity and specificity of the iELISA was superior to that of gELISA and cELISA. The correlation and agreement between the iELISA and the gELISA were relatively good (*R*i/g 2 = 0.65 and ĸ i/g = 0.66, respectively), while the correlation and agreement of these two ELISAs with cELISA were low (*R*i/c2 = 0.46, *R*g/c2 = 0.37 and ĸ i/c = 0.62, ĸ g/c = 0.42). In spite of using the same anti-odontocete IgG antibody, the iELISA was more specific than were the IF and DB tests. An association between high antibody titers and the presence of neurological symptoms in dolphins was observed. The prediction is that iELISA based on broadly cross-reacting anti-dolphin IgG antibody would be a reliable test for the diagnosis of brucellosis in odontocetes, including families not covered in this study. Pdf copies are available on request: [email protected] Best regards, Gabriela Hernández Mora Investigation Program in Tropical Diseases (PIET) Universidad Nacional, P.O.Box 304-3000, Heredia Costa Rica www.fundacionketo.org
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