How is the second card configured?

Does it have its own IP on a seperate, unique private subnet shared
with the testtool?

Go to a command prompt window and give us the output of the following
two commands:

ipconfig /all
route print

Does only LabVIEW fail to communicate, or is the failure at the OS
level? Can you ping the testtool?

Do you have any kind of personal firewall software installed?

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