If you are creating a pulse in software somehow, then you can use
Pulse Measurements.vi that's on the Analyze>Waveform Measurements
palette. This will require that the input is of the waveform data type
with signal and timing information. There's several timing functions
on the time & Dialog palette that you could probably use. Check out
Tick Count and Elapsed Time. If you could provide more details on what
exactly you need to do, we could provide more help.

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