Hi Atila, Lambada was not able to properly identify the membrane reference atoms and you get that error message when it tries to calculate the membrane centre in the Z-axis.
Although I'm not an expert I would recommend you to use an index file for the protein and the membrane (options -n1 and -n2, type lambada -h). In my case this was the solution to that problem, but my system is simpler than yours. -- Catarina Santos Research Student Molecular Simulation Group Instituto de Tecnologia Química e Biológica Universidade Nova de Lisboa Av.da República, Apartado 127 2781-901 Oeiras, Portugal -- gmx-users mailing list gmx-users@gromacs.org http://lists.gromacs.org/mailman/listinfo/gmx-users * Please search the archive at http://www.gromacs.org/Support/Mailing_Lists/Search before posting! * Please don't post (un)subscribe requests to the list. Use the www interface or send it to gmx-users-requ...@gromacs.org. * Can't post? Read http://www.gromacs.org/Support/Mailing_Lists