Dear all,
I'm trying to simulate a protein with artificial six histidine residues added on the N-terminal. As all would know very well, it is common to have His tag attached on protein for purification reasons, and is thought to affect the protein's activity minimally. I'm suspecting that in my case this His tag might affect the protein's activity greatly, and want to add His tag to my protein model. An NMR structure of the protein without His tag is available on PDB, but is of course without His tag. I have tried several programs to attach six histidines to the N-terminus, but failed as the chain is no longer recognized as a protein after the modification. I would be grateful if anyone could give me a clue on this. Many thanks in advance. Sincerely, Jong Wha Lee -- gmx-users mailing list gmx-users@gromacs.org http://lists.gromacs.org/mailman/listinfo/gmx-users * Please search the archive at http://www.gromacs.org/Support/Mailing_Lists/Search before posting! * Please don't post (un)subscribe requests to the list. Use the www interface or send it to gmx-users-requ...@gromacs.org. * Can't post? Read http://www.gromacs.org/Support/Mailing_Lists
