Hi I am using a dimer for my simulation system which is connected by 2 disulfide bonds. After energy minimization for even few cycles, the disulfide bond breaks which is not expected. i have applied amber99 force field and prepared the system. while i tried with another force field in Gromacs which did not worked at all. also i have tried by editing specbond.dat as well but of no use. can any one share/discuss some ideas how to fix this.
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