Dear justin Thanks for tutorial on membrane protein simulation . I am following your tutorial, everything is fine but problem came in concatanation . After orrienting the protein by using editconf I did the concatanation process everything is fine but the file which I got after concatanation i.e system.gro when I viewed this file in pymol the insertion is totally wrong the protein inserted parallel to bilayer hydrophobic core it is totally wrong I need protein should inserted perpendicular to lipid bilayer i.e from one side polar head of lipid to another side by crossing hydrophobic core of lipid .
can u suggest me right method to insert my protein into lipidbilayer in proper orientation before using inflategro methedology. I am just stuck on that point . I am eagerly waiting for your reply. Thanks a lot in advance. NITU SHARMA SCHOOL OF LIFE SCIENCES JAWAHERLAL NEHRU UNIVERSITY NEW DELHI, INDIA
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