Hi Sam,

I think this part ISNUMBER(--MID(A3,6,4)) will allow entry with 3 spaces 
before the fourth digit :-) , like

ABCDE   1D

Hi Vijayendra,

This is my try. Firstly define 3 names to avoid INDIRECT function.

Name: *Alpha*
On refers to: 
={"A";"B";"C";"D";"E";"F";"G";"H";"I";"J";"K";"L";"M";"N";"O";"P";"Q";"R";"S";"T";"U";"V";"W";"X";"Y";"Z"}

Name: *Row*
Refers to: ={1;2;3;4;5}

Name: *Col*
Refers to: ={6,7,8,9}

Then use this formula in Data Validation >> Custom. Make sure you have 
unselect *Ignore blank*

=SUMPRODUCT(ISNUMBER(MATCH(MID(C2,Row,1),Alpha,0))*ISNUMBER(MID(C2,Col,1)+0)*ISNUMBER(MATCH(MID(C2,10,250),Alpha,0)))=20

HTH
Haseeb

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