Hello all-- I'm looking for some advice on surface-sterilization of seedling root systems. I am transplanting 2-month-old Douglas-fir (Pseudotsuga menziesii) seedlings and have noticed light molding along the root system 1-2 days post-transplantation into a nutrient-rich medium. (The root systems looked clean upon examination before transplantation.)
I was wondering if a gentle rinse in bleach or hydrogen peroxide might kill off this contaminant, without damaging the seedlings. Has anyone had any experience with this before, or, even better, have a protocol that they recommend? Thanks very much in advance for your help! Best, Holly Holly V. Moeller Ph.D. Candidate, Ecology and Evolution Stanford University Department of Biology [email protected]
