REQUEST FOR ASSISTANCE - We are two PhD students at Evolutionary Genetics Group, Groningen University (The Netherlands). Our names are Silvia Paolucci and Maartje Giesbers. We are in the third year of our 4 years PhD project. We study genetics of adaptation using Nasonia parasitoid wasps as study organisms. One of our research goals is to investigate intra- and inter-specific variation for certain adaptive traits in natural populations. We are currently planning our field work season in North America in late spring-summer 2011. We would like to ask your kind help in planning our field work because we collect the wasps in bird nest boxes. Female Nasonia wasps lay eggs on fly pupae; wasp larvae develop inside the host and emerge as adults after 15-20 days. These wasps can be collected in bird nests because their hosts (blowflies and other fly species) are common nest inhabitants. Nestling birds become infested by blowflies when adult flies lay eggs directly on nestlings or on nest material near nestlings. Fly eggs hatch within 24-48 hours after they are laid and the larvae must feed immediately by sucking birds'; blood. Pupation usually occurs shortly after nestlings have fledged. It is in this developmental stage (pupal stage) that flies are parasitized by Nasonia. We typically collect the nest material of various bird species after young birds have fledged. The nest material is then sorted and fly pupae are isolated and maintained until wasps or flies emerge. We then use the wasps for our experiments. The best timing to sample wasps is between one and two weeks after the birds' fledging. We would like to sample in the coming spring-summer in North America and we are looking for bird nests trail holders that like to help us in our field work. Please note that since wasp collection has to take place after fledging, birds are not disturbed and empty nests are removed from nest boxes. Contact SILVIA PAOLUCCI, (EM: <file:///C:/Users/David/AppData/Local/Temp/ma ilto:[email protected]>[email protected], PH: +31 (0)50 363 2336).

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