Hi, I have done oxidative stress research with tads. This was preliminary work which I would like to take further (lots of directions to go).
The citation is of the work was: Jones, L., D.R. Gossett, S.W. Banks, and M.L. McCallum. 2010. The effect of paraquat on the antioxidant defense system of the American Bullfrog (Rana catesbeiana). Journal of Herpetology 44(2):222-228. You can download the pdf to this paper at: http://www.herpconbio.org/McCallum/reprints.htm On Tue, Nov 9, 2010 at 6:06 AM, Eric Flores <[email protected]> wrote: > Hi all > > I am requesting your help related to analysis of SOD, lipid peroxidation and > protein carbonyl assay to metamorphed froglets. > My problem is that I have samples of entire frogs frozzen under -80°C and > the SOD and lipid peroxidation require to homogenate the frogs using > different buffers to run the analysis. Moreover the protein assay kit > analyses mention that samples containing significant haemoglobin may > interfere with the assay. > > I will really appreciate any suggestion and advice on how to proceed without > compromise the results of the analysis. > > Thanks > > Eric > -- Malcolm L. McCallum Managing Editor, Herpetological Conservation and Biology "Peer pressure is designed to contain anyone with a sense of drive" - Allan Nation 1880's: "There's lots of good fish in the sea" W.S. Gilbert 1990's: Many fish stocks depleted due to overfishing, habitat loss, and pollution. 2000: Marine reserves, ecosystem restoration, and pollution reduction MAY help restore populations. 2022: Soylent Green is People! Confidentiality Notice: This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message.
