Andreas Tille <[email protected]> writes: > blastn [-h] [-help] [-import_search_strategy filename] [...] > DESCRIPTION > Nucleotide-Nucleotide BLAST 2.10.1+ [...] > Error: Unknown argument: "query_gencode"
Please try simply dropping -query_gencode (and its argument) here -- because blastn performs direct nucleotide-nucleotide comparisons, it doesn't care about genetic codes, and upstream evidently limited this flag's use to the one tool for which it would be meaningful: tblastx. (Likewise, only tblastn and tblastx support -db_gencode these days.) Thanks for checking! -- Aaron M. Ucko, KB1CJC (amu at alum.mit.edu, ucko at debian.org) http://www.mit.edu/~amu/ | http://stuff.mit.edu/cgi/finger/[email protected]
