Hi Andreas,

Thanks for you work. I answer your questions as bellow:

- some small fixes:
https://anonscm.debian.org/viewvc/debian-med/trunk/packages/metaphlan2/trunk/debian/patches/fix_sequence.patch?view=markup

    -> fixed
- some spelling issues https://anonscm.debian.
org/viewvc/debian-med/trunk/packages/metaphlan2/trunk/debian/patches/spelling.patch?view=markup

- Tin can also provide more info about the binary data in db_v20. The files
ending with "bt2" are created using a script in the Bowtie2 package
(bowtie2-build) using a sequence file Tin can provide (it can also be
recovered from the bt2 files with bowtie2-inspect if I remember well).
  As Nicola said, those files in db_v20 are created with bowtie2-build
using a sequence file and you can recover the sequence file by:

bowtie2-inspect metaphlan2/db_v20/mpa_v20_m200 > metaphlan2/markers.fasta

If you want to rebuild them, the command is:

bowtie2-build metaphlan2/markers.fasta metaphlan2/db_v21/mpa_v21_m200


- For the mpa_v20_m200.pkl Tin can also provide the uncompressed python
object (or he can provide a couple of lines of code to uncompress it?)
   It is python dictionary and can be read as:

import cPickle as pickleimport bz2
db = pickle.load(bz2.BZ2File('db_v20/mpa_v20_m200.pkl', 'r'))


You can have more information about them at:
https://bitbucket.org/biobakery/metaphlan2#markdown-header-customizing-the-database

In addition, some files were changed the names:
   - metaphlan2_strainer.py -> strainphlan.py
   - strainer_src -> strainphlan_src
   - strainer_tutorial -> strainphlan_tutorial

Some source files were updated as well.
Please let me know if you need other information.

Thanks,
Tin

On Wed, Aug 3, 2016 at 3:38 PM Andreas Tille <[email protected]> wrote:

> Hi Nicola,
>
> thanks for your answer.
>
> On Tue, Aug 02, 2016 at 04:32:31PM +0000, Nicola Segata wrote:
> > Hi Andreas,
> >  sorry for the delay in replying. I did get your last two emails but it
> > seems the fist one (On Mon, Jul 25, 2016 at 09:45:57PM) never arrived.
>
> Hmmm, sad that there seems to be some mail loss.
>
> > Tin can also provide more info about the binary data in db_v20. The files
> > ending with "bt2" are created using a script in the Bowtie2 package
> > (bowtie2-build) using a sequence file Tin can provide (it can also be
> > recovered from the bt2 files with bowtie2-inspect if I remember well).
> >
> > For the mpa_v20_m200.pkl Tin can also provide the uncompressed python
> > object (or he can provide a couple of lines of code to uncompress it?)
>
> Anything that qualifies as source would be really welcome.  If the
> generation of the binary from this source does not make a big effort (in
> terms of "takes way longer than 1 hour on a decent build machine")
> generating the binaries would be really prefered.
>
> > For the LEfSe package I just added the license in the bitbucket
> repository.
> > For the description, I think you can use the following page:
> > https://bitbucket.org/biobakery/biobakery/wiki/lefse
> > Does it sound like an appropriate description for the package?
>
> I found this after I've sent my mails - thanks for confirming that this
> is the correct description.  I've just uploaded the package to the
> Debian new queue.
>
> > Let me know if you have other questions or if I missed answering to other
> > emails.
>
> If Tin will answer the binary data issue above I have no further
> questions and do not remember any unanswered e-mails.
>
> > thanks so much for your work!
>
> You are welcome
>
>       Andreas.
>
> --
> http://fam-tille.de
>

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