I also have the same problem. Log: $ cat x.fq @C17VNACXX121020:6:1101:10000:52894/2 AGACCTCGCTACCCGCCAGCGGGGA + CCCFFFFFHHGHHJJJJJJJJJJJA $ fastqc x.fq Started analysis of x.fq Analysis complete for x.fq Failed to process file x.fq java.lang.NullPointerException at uk.ac.babraham.FastQC.Report.HTMLReportArchive.startDocument(HTMLReportArchive.java:170) at uk.ac.babraham.FastQC.Report.HTMLReportArchive.<init>(HTMLReportArchive.java:60) at uk.ac.babraham.FastQC.Analysis.OfflineRunner.analysisComplete(OfflineRunner.java:157) at uk.ac.babraham.FastQC.Analysis.AnalysisRunner.run(AnalysisRunner.java:108) at java.lang.Thread.run(Thread.java:722) $ fastqc -version FastQC v0.10.1
--- Best regards, Alexey Sergushichev, University ITMO, St. Petersburg, Russia