Dear Colleagues, Please see the following job posting for a postdoctoral fellowship at Purdue University.
Thanks Chitta Postdoctoral Associate – Atypical ubiquitination in host-pathogen interactions About Das Lab ( https://www.chem.purdue.edu/das/ ):The broad research interest of the group centers around protein ubiquitination and its relationship with bacterial and viral pathogens. Specifically, understanding mechanism of action of ubiquitin ligases, deubiquitinases (DUBs) and ubiquitin modifying enzymes of both prokaryotic and eukaryotic origin. The fact that viruses and bacteria manipulate our ubiquitination pathways has been known for at least two decades, with several well characterized examples of pathogens using enzymes or substrate adaptors to co-opt our ubiquitination pathways. We have recently identified atypical modes of ubiquitination and deubiquitination catalyzed by bacterial effectors. We wish to unravel mechanism of action of these enzymes, including regulation of catalytic activity and substrate selection using biochemical, biophysical, molecular biological techniques and integrative structural approaches such as cryo-EM, crystallography, cross-linking mass spectrometry, and proteomics to answer fundamental questions on how pathogens manipulate various ubiquitin-related host signalling pathways. Example publications related to this work can be found here: Kenny S, Iyer S, Gabel C, Tegenfeldt M. Demarco A, Hall MC, Chang L, Davisson V, Vande Pol S, Das C. Structure of E6AP in complex with HPV16-E6 and p53 reveals a novel ordered domain important for E3 ligase activation. Structure. 2025 Mar 6;33(3):504-516.e4. doi: 10.1016/j.str.2024.12.013. Epub 2025 Jan 15. Zhang Z, Fu J, Rack J, Li C, Voorneveld J, Filippov D, Ahel I, Luo ZQ, Das C. Legionella metaeffector MavL reverses ubiquitin ADP-ribosylation via a conserved arginine-specific macrodomain (2024) Nature Communications 15 (2452): https://doi.org/10.1038/s41467-024-46649-2. Zhang Z, Patel R, Zhao-Qing Luo, Das C. Cryo-EM Detection of AMPylated Histidine Implies Covalent Catalysis in AMPylation Mediated by a Bacterial Effector. Journal of Molecular Biology 2025 Feb 1;437(3):168917.doi: 10.1016/j.jmb.2024.168917. Resources: Das lab is well equipped for carrying out all molecular biology and biochemical experiments. In addition to the resources in-house, the Das group members also have access to campus-wide facilities such as proteomics facility, cryo-EM facility (Titan Krios G4 microscopes), Markey Center for Structural Biology (which offers access to crystal growth facilities, temperature-controlled crystallization chambers and the Mosquito X1 Crystallization Robot), Bindley Biophysics Core and Purdue University of Drug Discovery. Application Process: We are seeking a highly motivated recent Ph.D. graduate with a strong background in molecular biology and biochemistry. Interested candidates should submit a CV, a cover letter detailing research interests and accomplishments, and contact information for three references to [email protected]<mailto:[email protected]>. We encourage candidates from diverse backgrounds to apply. Purdue University is an equal opportunity employer. ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
