I discovered recently that real chemists - the hardcore process types - know 
and love isocratic solvents.  The one I learnt it from, had no trouble quickly  
demonstrating why.

So... we've been missing a trick.

(I'm aware this is a slightly more vague answer than you needed.)

Frank

Sent from tiny silly touch screen
________________________________
From: Gloria Borgstahl <[email protected]>
Sent: Tuesday, 9 September 2025 20:55
To: [email protected]
Subject: [ccp4bb] SMI protein target stall structures

Hello Friends,  I am interested in finding out what protocols people are using 
to work with SMIs dissolved in 100% DMSO.  What steps do you take to get the 
compound into the minimal DMSO (2-5%)?  If soaking do you try to get it 
dissolved into the crystallization reservoir solution?  Thank you!
Asking for a friend,  Gloria

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