Hmmm - of course you are free to contact the authors and explain what you
have found. There are many sub-optimal structures deposited and the pdb
tries to validate them ; more strictly now than in the past .

 In this case I would refer to the pdb-redo site and check if that has
corrected some errors .
Then if necessary, and if you want to use the structure in some way,
re-refine it yourself. That is the beauty of the PDB deposition - we can
all access and use the data for our ends.
Thank you - PDB ancestors!
Eleanor

On Fri, 30 May 2025 at 22:30, Nikolas <[email protected]> wrote:

> *enters the BB and kneels in reverence*
>
> Dear BB,
>
> Recently, delving among papers and structures for a current project, I
> came across a deposited structure of interest that is terribly modeled. In
> the PDB resolution is 2.00 Å (R/Rfree=0.19/0.22) and the quality indicators
> are not terrible but upon opening it and checking the maps there are some
> severe mistakes in the modeling.
> For instance, a full chain that is in the negative Fo-Fc while positive
> density is present, some density that is quite clear for SO4 molecules
> (buffer) modeled as water and density for water molecules neglected. Some
> parts seem like “coot:runwater” has been used and not checked. The
> catalytic site is well defined.
>
> Now, I’ve started my journey in crystallography not too long ago and I’ve
> got many years, structures to solve and things to learn, and I know that
> sometimes the dataset “is what it is” but these seems quite big mistakes.
> Especially because I’ve worked with such proteins before and I know the
> systems.
>
> My question thus is: would it be right to contact the author of this
> structure and point to these issues? What’s the best way to address such
> things, possibly without coming off as obnoxious?
>
> Thank you for your wisdom.
>
> Cheers,
> Nikolas
>
> *bows and leaves the BB*
>
>
>
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