Hello everyone I am planning to teach a Cryo-ET course module for undergraduate students at an introductory to intermediate level next year. The course will cover image processing and 3D reconstruction, including tilt series alignment, segmentation, and subtomogram averaging, aiming to achieve a resolution of 3–5 Å for 500 kDa–2000 kDa protein complexes (e.g., ribosomes, viruses, microtubules, RuBisCO) using raw data and tilt series obtained from the EMDB repository.
While I have extensive experience in single-particle reconstruction, my knowledge of Cryo-ET is primarily theoretical. One of the key challenges is that Cryo-ET data processing is not a straightforward, single-software process—it requires multiple software tools for different stages, and sometimes even multiple tools for a single step. Could anyone recommend a streamlined Cryo-ET workflow with a minimal set of well-established software tools for these steps that can cover the entire reconstruction process, from raw tilt series to high-resolution structures? My goal is to first gain hands-on experience with the workflow and then use it as the foundation for teaching the students next year. Any guidance to design this teaching module would be greatly appreciated Best Firdous ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
