Simply transfer the crystals into your stabilization or soaking buffer using sitting drop or hanging drop plates. I transferred a few drops to remove the oil, then soaked the ligand, and it worked. You might encounter some oil droplets, but we were able to manage and successfully collect data. This method was used for DNA gyrase. Or set up co-crystallisation in microbatch plate. Thanks Kannan
On Mon, 17 Mar 2025 at 12:14, CENGIZ KAAN FERAH < 0000a9b927892eef-dmarc-requ...@jiscmail.ac.uk> wrote: > Hello everyone, > > I'm currently working on a protein and I did solve the apo structure of > the enzyme. I'm trying to get the ligand bound form of the enzyme. The > ligand is L-alanine. How should I soak with the ligand? I'm doing > microbatch under oil with a terasaki plate without any machinery. > > Thank you. > Best Regards. > > ------------------------------ > > To unsubscribe from the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 > ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
