Dear all, happy summer! I searched in many ways but could not find relevant references easily so I'm hoping you could help.
For TSA assays on a Cys-containing protein (drug target) that has just gone through a covalent adduction reaction with a compound carrying a Michael acceptor covalent warhead, we would like to stop the further progression of the covalent adduction before subjecting the reaction mixture to TSA analysis. It is my understanding that people usually use DTT to deplete the excess covalent compound - do you happen to come across such a scientific reference? Thank you so much in advance, -yong ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
